01822nas a2200217 4500008004100000245010100041210006900142260001400211300001200225490000600237520116600243653001601409653000901425653001501434653002101449653002101470100002801491700001601519700002001535856004901555 2017 eng d00aAntioxidant Activity and Isolation of Xanthine Oxidase Inhibitor from Ruellia tuberosa L. Leaves0 aAntioxidant Activity and Isolation of Xanthine Oxidase Inhibitor cJuly 2017 a607-6100 v93 a

Introduction: The leaves of Ruellia tuberosa L. have been known to demonstrate strong antioxidant and xanthine oxidase (XOD) inhibitory activities. The aim of this study was to isolate antioxidant and XOD inhibitor from the leaves of the plants. Methods: Isolation of antioxidant and XOD inhibitor were conducted using chromatography techniques. The structure of the isolated compound was elucidated by spectroscopic methods. Results: In this study, a flavonoid was isolated and characterized as methoxylated flavonoid based on the spectral data including UV, IR, GC-MS, and NMR. The compound demonstrated DPPH free radical scavenging activity with IC50 of 28.79 μg/ml, and XOD inhibitory with IC50 of 0.67 μg/mL. Conclusion: The isolated compound was determined as 5-hydroxy-3,7-dimethoxy-2-(4-((3S,4S,5S,6R)-4,5, 6-trihydroxy-2(hydroxymethoxy)-tetrahydro-2H-pyrane-3-iloxy) phenyl)-4H-chromen-4-on or camarosids. The isolated compound demonstrated strong DPPH free radical scavenging and XOD inhibitory activity. 

10aAntioxidant10aDPPH10aFlavonoid.10aRuellia Tuberosa10aXanthine Oxidase1 aAhmad, Aktsar, Roskiana1 aElya, Berna1 aMun’im, Abdul u/files/pj-9-5/10.5530pj.2017.5.96/index.html