02520nas a2200253 4500008004100000245012500041210006900166260001300235300001200248490000600260520174900266653001802015653001702033653002502050653000902075653001802084100001802102700003002120700001702150700002302167700001502190700002302205856003802228 2017 eng d00aSimultaneous-HPLC Quantification of Phenolic Acids in Traditionally used Ayurvedic Herb Diplocyclos palmatus (L.) Jeffry0 aSimultaneousHPLC Quantification of Phenolic Acids in Traditional cMay 2017 a483-4870 v93 a

Introduction:The present study deals with the simultaneous HPLC-quantification of phenolic acid(s) in the aerial parts of Diplocyclos palmatus (Cucurbitaceae) and evaluation of their bioactivity potential through in vitro antioxidant assay’s. Method: The HPLC elution was done using C18 column using gradient (binary phases) solvent system at a flow rate of 0.6 ml/min. Total phenolic and, flavonoid contents were determined and the antioxidant potential was estimated by four assay’s viz. DPPH radical scavenging assay, ferric reducing power assay, total antioxidant capacity and 2-deoxy ribose assay. Results: The species is rich in three phenolic acids, among which gallic acid (1708 ug/g) is in maximum concentration followed by caeffic acid (437 ug/g) and protocateuchic acid (337.7 ug/g). Total phenolic content was higher (10.5 mg/g) than flavonoid content (3.78 mg/g) and TAC was found at 0.137 mg/g ASE (ascorbic acid equivalent). IC50 of D. palmatus extract for scavenging of hydroxyl radical by 2-deoxy ribose and DPPH was at concentration of 125.61 ± 0.834 (μg/ml) and 353.71 ± 0.663 (μg/ml) respectively. In vitro antidiabetiv potential, via inhibition of alpha amylase enzyme through starch iodine and 3,5- DNS assay reveals the IC50 of extract at 146.31 ± 0.415 ug/ml and 286.23 ± 0.671 ug/ ml respectively. Conclusion: The species (aerial part) was rich in phenolic acid with potential bioactivity, identified leads will be useful in further chemical characterization and pharmacological validation.

10aAnti diabetic10aAnti oxidant10aDiplocyclos palmatus10aHPLC10aPhenolic acid1 aMisra, Ankita1 aShukla, Pushpendra, Kumar1 aKumar, Bhanu1 aNiranjan, Abhishek1 aRawat, AKS1 aSrivastava, Sharad u/files/PJ-9-4/10.5530pj.2017.4.78