02562nas a2200217 4500008004100000245010900041210006900150260001800219300001200237490000600249520187200255653002202127653001602149653000902165653004202174653002802216100002702244700001602271700002202287856003502309 2017 eng d00aInhibition of Alpha-Glucosidase and Antioxidant Test of Stem Bark Extracts of Garcinia fruticosa Lauterb0 aInhibition of AlphaGlucosidase and Antioxidant Test of Stem Bark cFebruary 2017 a273-2750 v93 a

Introduction: Diabetes mellitus (DM) is one of the global health emergencies that characterized by high blood glucose levels (hyperglycemia). Type 2 DM is the most common type in diabetic populations. Inhibition of alphaglucosidase can ameliorate postprandial hyperglycemia that occurs in patients with type 2 DM. Adding antioxidants to the therapy of DM is intended to reduce complications caused by oxidative stress. Some species of Garcinia have been proven to inhibit alpha-glucosidase and have antioxidant activity, but there is no research on Garcinia fruticosa Lauterb. Therefore, the aims of this research were to determine the activity of Garcinia fruticosa Lauterb. stem bark in inhibiting alpha-glucosidase and as an antioxidant. Methods: In this research, the Garcinia fruticosa Lauterb. stem bark was dried, grinded, and extracted by multistage maceration using n-hexane, ethyl acetate, and methanol. Inhibition of alpha-glucosidase test has been done in vitro on concentrated extracts and measured by microplate reader at 400 nm. The antioxidant test has been done using DPPH scavenging method and was measured by microplate reader at 519 nm. Results: Ethyl acetate extract is the most active extract for both test. IC50 values for inhibition of alpha-glucosidase test are 20.18 μg/mL that is more active than standard (acarbose) which has IC50 value 141.55 μg/mL. Meanwhile, IC50 value from an antioxidant test is 8.93 μg/mL that is not more active than standard (quercetin) which has IC50 value 2.51 μg/mL. Conclusion: Phytochemical screening shows that the ethyl acetate extract contains alkaloids, flavonoids, glycosides, saponins, and tannins.

10aAlpha-glucosidase10aAntioxidant10aDPPH10aGarcinia fruticosa Lauterb. Stem bark10aPhytochemical screening1 aZahratunnisa, Nusaibah1 aElya, Berna1 aNoviani, Arikadia uhttp://phcogj.com/fulltext/313