02218nas a2200229 4500008004100000245013500041210006900176260001800245300001200263490000600275520152300281653002101804653001601825653001401841653000901855653003201864653001701896100001901913700001601932700000501948856003501953 2017 eng d00aAntioxidant Activity and Lipoxygenase Enzyme Inhibition Assay with Total Flavonoid Content from Garcinia hombroniana Pierre Leaves0 aAntioxidant Activity and Lipoxygenase Enzyme Inhibition Assay wi cFebruary 2017 a267-2720 v93 a

Objective: Garcinia hombroniana Pierre leaves extract have been known to contain flavonoid, but it has not been known yet for its antioxidant activity and inhibition of lipoxygenase activity. This study aims to determine antioxidant activity and inhibition of lipoxygenase activity of G. hombroniana leaves extract. Method: Antioxidant activity tested by using FRAP (Ferric Reducing Antioxidant Power) method and inhibition of lipoxygenase activity using baicalein as the positive control. Total flavonoid assay is also quantitatively done by AlCl3 colorimetric method on the most active extract using quercetin as the positive control. Results: The test result showed that the n-hexane, ethyl acetate and methanol extract of G. hombroniana Pierre leaves have antioxidant activity which showed by EC50 value consecutively are 36.260; 2.969; and 7.416 μg/mL, and also can inhibit lipoxygenase activity which showed by IC50 value consecutively are 2.052; 0.134; and 1.314 μg/mL. Ethyl acetate extract of G. hombroniana Pierre leaves has the most active antioxidant activity and inhibition of lipoxygenase activity. Total flavonoid content of ethyl acetate extract of G. hombroniana Pierre leaves is 42.004 mg QE/g sample. Conclusion: Garcinia hombroniana Pierre leaves extract has antioxidant activity and can inhibit lipoxygenase activity.

10aAntiinflammation10aAntioxidant10aFlavonoid10aFRAP10aGarcinia hombroniana Pierre10aLipoxygenase1 aMarlin, Shinta1 aElya, Berna1 a uhttp://phcogj.com/fulltext/312