02517nas a2200265 4500008004100000245018000041210006900221260001800290300001200308490000600320520167200326653000801998653001602006653001202022653002202034653002402056653002102080653001902101100002702120700002602147700000502173700001802178700002002196856003502216 2017 eng d00aEffect of Gamma Irradiation on Angiotensin Converting Enzyme Inhibition, Antioxidant Activity, Total Phenolic Compound and Total Flavonoid of Peperomia pellucida Herbs Extract0 aEffect of Gamma Irradiation on Angiotensin Converting Enzyme Inh cFebruary 2017 a244-2480 v93 a

Introduction: Peperomia pellucida L. Kunth has been reported to have some biological activities such as antihypertensive and antioxidants. Herbal materials susceptible to contamination during processing and storage which can shortens their shelf life. Gamma-irradiation has been used as a method for preservation. Methods: This research aimed to analyze the effect of gamma-irradiation on inhibition activity of angiotensin converting enzyme (ACE), antioxidant activity, total phenol content, total flavonoid, and thin layer chromatography profiles of P. pellucida L. Herbs extract. The extract was irradiated with 60Co gamma rays at 2.5; 5; 7.5; dan 10 kGy. Results: Irradiation up to 10 kGy did not change ACE inhibitory activity and TLC profile. No significant differences were noted in the inhibition activity of ACE and the type of chromatogram profiles between non-irradiated extract and irradiated extracts up to a dose of 10 kGy while total flavonoids showed a significant decrease. In addition, total phenolic content and antioxidant activity showed a significant increase of extracts were irradiated up to 5 kGy and decrease at dose 7,5 kGy and 10 kGy. Gamma-irradiation up to 10 kGy didn’t affect the activity of ACEinhibitor in-vitro while significant difference (P <0.05) of antioxidant activity, total flavonoids and phenolic content of the extract P. Pellucida. Conclusion: In conclusion, gamma - irradiation can be used as a preservation method for ethanol extract P. Pellucida L. Kunth herbs.

10aACE10aAntioxidant10aExtract10aGamma-irradiation10aPeperomia pellucida10aTotal flavonoids10atotal phenolic1 aAdhitia, Anies, Monica1 aOctaviani, Alisa, Nur1 a1 aBasah, Katrin1 aMun’im, Abdul uhttp://phcogj.com/fulltext/308