02499nas a2200265 4500008004100000245015300041210006900194260001500263300001000278490000700288520164000295653002201935653001001957653002001967653001401987653001902001653003002020100002302050700002102073700002902094700002402123700002402147700002202171856004002193 2026 eng d00aAnti-inflammatory Activity, Toxicity Assessment and GC-MS Chemical Profiling of Cha-Nu-Ruk Traditional Thai Herbal Poultice for Knee Pain Management0 aAntiinflammatory Activity Toxicity Assessment and GCMS Chemical  cApril 2026 a31-350 v183 a

Introduction: Thai Traditional herbal medicine employs multi-herb formulations for managing inflammatory conditions. This study investigates the anti-inflammatory properties, cytotoxicity, and chemical composition of Cha-Nu-Ruk, an eight-herb traditional poultice formulation for knee pain. Methods: Eight medicinal plants (Tiliacora triandra, Thunbergia laurifolia, Azadirachta indica, Clinacanthus nutans, Pandanus amaryllifolius, Justicia gendarussa, Sida acuta, and Zingiber cassumunar) were extracted with 70% ethanol. Anti-inflammatory activity was evaluated through nitric oxide inhibition assay using Jurkat cells. Cytotoxicity was assessed via MTT assay. Chemical profiling employed GC-MS analysis. Results: Individual plant extracts demonstrated variable anti-inflammatory activity (NO inhibition: 28.52-73.80%) with Clinacanthus nutans showing highest potency. The combined eight-herb formulation yielded 11.82±0.41% extraction efficiency with 26.2% NO inhibition. GC-MS analysis identified 21 compounds, with DMPBD (19.13% relative abundance) and β-sitosterol (1.13%). Cytotoxicity evaluation revealed acceptable safety profiles (>80% cell viability) for most extracts. Conclusions: While individual herbs showed promising anti-inflammatory potential, the traditional eight-herb combination did not demonstrate synergistic enhancement. Chemical standardization revealed significant batch-to-batch variability, highlighting the need for optimized formulation ratios and quality control protocols.

10aAnti-inflammatory10aGC-MS10aHerbal poultice10aKnee pain10aPhytochemistry10aThai traditional medicine1 aDenchai, Rattazart1 aNualkaew, Somsak1 aLaovachirasuwan, Pornpun1 aPornsiri, Nopphawan1 aJudprakop, Chonlada1 aRattana, Surapong uhttps://www.phcogj.com/article/2588