02528nas a2200253 4500008004100000245010100041210006900142260001800211300001200229490000700241520173700248653001501985653001502000653002502015653002802040653001502068653002602083100003102109700002402140700002602164700002202190700002202212856004002234 2025 eng d00aFree Radical Scavenging and Anticancer Activities of Methanolic Twig Extract of Annonaceae Plant0 aFree Radical Scavenging and Anticancer Activities of Methanolic  cDecember 2025 a362-3650 v173 a

Introduction: The Annonaceae family represents a promising source of bioactive compounds with potential therapeutic applications. This study aimed to comprehensively evaluate the antioxidant and anticancer potential of methanolic twig extracts from seven Annonaceae species. Methods: Twig specimens from Cananga latifolia, Goniothalamus elegans, Goniothalamus tamirensis, Melodorum fructicosum, Polyalthia dubia, Polyalthia cerasoides, and Uvaria fauveliana were subjected to standardized methanolic extraction. Antioxidant activity was assessed using DPPH radical scavenging assay. Anticancer potential was evaluated through Sulforhodamine B assay against three human cancer cell lines and normal human dermal fibroblasts at 25 μg/mL concentration. Results: Goniothalamus elegans demonstrated exceptional antioxidant activity (IC50 = 5.62 ± 1.21 μg/mL) comparable to ascorbic acid. In anticancer evaluation, G. elegans exhibited remarkable cytotoxicity against MCF-7 (95.19 ± 0.62%) and HeLa (94.46 ± 1.69%) cancer cells while demonstrating exceptional selectivity with minimal toxicity toward normal cells (19.72 ± 1.19%). Melodorum fructicosum showed highest activity against HT-29 colon cancer cells (75.84 ± 1.57%). Conclusions: Goniothalamus elegans demonstrated exceptional dual bioactivity with both potent antioxidant properties and selective anticancer effects. The remarkable selectivity indices and broad-spectrum anticancer activity suggest significant clinical potential. These findings provide scientific validation for traditional medicinal uses of Annonaceae species.

10aAnnonaceae10aAnticancer10aantioxidant activity10aCancer cell selectivity10aDPPH assay10aGoniothalamus elegans1 aWongtangtintharn, Sawitree1 aMcCloskey, Sirirath1 aThiwthong, Rungruedee1 aThongpairoj, U-sa1 aRattana, Surapong uhttps://www.phcogj.com/article/2580