02214nas a2200265 4500008004100000245008800041210006900129260001800198300001200216490000600228520143600234653003401670653002501704653002401729653002001753653001901773100003101792700002101823700001701844700000501861700002201866700000501888700002001893856003501913 2017 eng d00aEffect of Gamma Irradiation on Suruhan (Peperomia pellucida (L.) Kunth) Herb Powder0 aEffect of Gamma Irradiation on Suruhan Peperomia pellucida L Kun cFebruary 2017 a239-2430 v93 a
Introduction: Peperomia pellucida (L.) Kunth is known as a raw material for herbal medicine; Preservation of herbs powder by gamma irradiation is reported to be able to keep products free from contamination. Methods: This study aims to evaluate the effect of gamma irradiation (0; 2.5; 5; 7.5; and 10 kGy) on the ACE inhibitory activity (ACE Kit – WST test kit method), antioxidant activity (DPPH radical scavenging method), total phenolic content (colorimetric method using Folin-Ciocalteu reagent), total flavonoid content (colorimetric method using AlCl3 and sodium acetate), and TLC profiling (silica gel F254 as the stationary phase and dichlormethane:methanol [92:8] as the mobile phase) of suruhan herb powder. Results: Results showed that the 2.5 kGy irradiation dose gave the smallest alteration in ACE inhibitory activity compared to others irradiated doses. Furthermore, the 5 and 7.5 kGy dose didn’t cause significant change (p>0.05) on antioxidant activity, total phenolic content, and total flavonoid content. Antioxidant activity was found to correlate with the total phenolic content but not with the total flavonoid content. Conclusion: Based on these finding, it is concluded that gamma irradiation can be used as a preservation method for P. pellucida herb powder.
10aAngiotensin converting enzyme10aantioxidant activity10aPeperomia pellucida10atotal flavonoid10atotal phenolic1 aYusuf, Mubarika, Sekarsari1 aWulandari, Intan1 aAmelia, Lili1 a1 aNoviani, Arikadia1 a1 aMun’im, Abdul uhttp://phcogj.com/fulltext/307