02159nas a2200217 4500008004100000245011900041210006900160260001800229300001200247490000700259520145600266653001501722653003201737100001901769700003201788700002701820700001501847700002001862700001901882856004001901 2025 eng d00aQuantification of Phenolics, Flavonoids, and In Vitro Antioxidant Activity in Rosella and Breadfruit Leaf Extracts0 aQuantification of Phenolics Flavonoids and In Vitro Antioxidant  cDecember 2025 a688-6980 v173 a

Indonesia is a country rich in biodiversity, with numerous plant species traditionally used in medicine. One such plant is breadfruit (Artocarpus altilis), whose leaves contain bioactive compounds such as flavonoids, saponins, tannins, quercetin, artocarpanone, and artoindonesianin. Another plant with high medicinal value is roselle (Hibiscus sabdariffa L.), a tropical plant from the Malvaceae family known for its rich phenolic content. Both plants are widely found across Indonesia and have potential as natural antioxidants. In this study, the dry extracts were prepared using the decoction method followed by spray drying. Phytochemical screening, total phenolic and flavonoid content analysis, and antioxidant activity tests were performed using standard in vitro methods. The DE2C extract (a combination of breadfruit leaves and roselle flowers) exhibited high total phenol content at 0.953 ± 0.005 g GAE/100 g and flavonoid content at 136.97 ± 5.050 μg QE/100 g. Meanwhile, the DE3C extract showed an IC₅₀ value of 540.55 ppm in the DPPH assay. Pearson correlation analysis showed a strong positive correlation between total phenolic content and antioxidant activity (R = 0.956, p < 0.05), while flavonoids also showed a moderate correlation (R = 0.502, p < 0.05). These results confirm that phenolic compounds play a key role in the antioxidant potential of the extract.

10aFlavonoids10aQuantification of Phenolics1 aRiasari, Hesti1 aFitriansyah, Sani, Nurlaela1 aHasanah, Siti, Uswatun1 aAulia, Mia1 aZavella, Khania1 aPadilah, Nurul uhttps://www.phcogj.com/article/2557