02634nas a2200229   4500008004100000245013200041210006900173260001600242300001200258490000700270520189400277653001502171653001302186653002602199653003002225653000802255100002302263700002702286700002802313700002302341856004002364       2025                        eng d00aAnalysis of Solvent Concentration Effect and Extraction Method on The Total Phenolic of Syzygium myrtifolium Walp. Leaf Extract0 aAnalysis of Solvent Concentration Effect and Extraction Method o  cAugust 2025  a461-4690 v173 a<p class="rtejustify"><strong>Background: </strong><em>Syzygium myrtifolium </em>Walp., an Indonesian ornamental plant, contains phenolic compounds with antioxidant, anti-inflammatory, and antibacterial activities. The demand for natural antioxidants is rising due to concerns over synthetic alternatives. <strong>Objectivity:</strong> To determine the total phenolic content (TPC) and antioxidant activity of <em>S. myrtifolium</em> leaf extracts obtained with various solvents (ethanol 60, 70, 80, 96% v/v, ethyl acetate, n-hexane) and two extraction methods (maceration and ultrasonic-assisted extraction, UAE), and to assess the effects of solvent polarity and extraction method. <strong>Methods: </strong>Leaves were extracted by maceration and UAE. TPC was measured via the Folin–Ciocalteu method (tannic acid equivalents, %) using UV–Vis spectrophotometry. Antioxidant activity was evaluated using the DPPH assay (IC₅₀). Phytochemical screening was also performed. <strong>Results:</strong> TPC (maceration): 60% ethanol = 18.76%, 96% ethanol = 13.64%, ethyl acetate = 8.33%, n-hexane = 0.83%. TPC (UAE): 60% ethanol = 20.02%, 96% ethanol = 14.04%, ethyl acetate = 10.13%, n-hexane = 0.92%. Solvent type/concentration and extraction method significantly affected TPC (<em>p</em> &lt; 0.05). UAE 96% ethanol showed the strongest antioxidant activity (IC₅₀ ≈ 16.03 ppm), ethyl acetate had moderate activity (≈67–76 ppm), and n-hexane the weakest (≈153–163 ppm). UAE 60% ethanol had the highest TPC but not the strongest activity (IC₅₀ ≈ 88.27 ppm), suggesting antioxidant potency depends on phenolic composition. <strong>Conclusion: </strong>UAE with 60% ethanol produced the highest TPC, while UAE with 96% ethanol exhibited the strongest antioxidant activity. Further profiling is needed to clarify the phenolic composition–activity relationship.</p>
10aMaceration10aphenolic10asolvent concentration10aSyzigium myrtifolium walp10aUAE1 aUtami, Novi, Fajar1 aSyarif, Rizky, Mulyana1 aIrawan, Chorry, Sundari1 aRamadhani, Shintia  uhttps://www.phcogj.com/article/2525