02434nas a2200205 4500008004100000245015000041210006900191260001600260300001200276490000700288520174900295653002402044653001602068653002202084653001502106653001902121100003202140700001602172856004002188 2025 eng d00aZinc Oxide Nanoparticle Green Synthesis Using Black Cumin Seed Aqueous Extract: Its Characterization and in vitro Anti- Hyperglycaemic Properties0 aZinc Oxide Nanoparticle Green Synthesis Using Black Cumin Seed A cAugust 2025 a434-4370 v173 a

Introduction: α-Amylase and α-glucosidase are carbohydrate metabolizing enzymes that are known to be involved in postprandial hyperglycaemia in diabetic patients. Objective: In a bid to source potent inhibitors of these enzymes, the study synthesized zinc oxide nanoparticles and evaluated the in vitro anti-hyperglycaemic activity. Materials and Methods: The seed aqueous extract of black Cumin (BC) was used as a capping and/or reducing agent to synthesize ZnO NPs from zinc acetate precursor. The BC-ZnONPs were characterized by microscopy (FESEM and HRTEM) and spectroscopy (UV-Vis and EDX) methods. In vitro anti-hyperglycaemic evaluation was based on α-amylase and α-glucosidase inhibition assays. Results: The BC-ZnONPs showed a spherical-to-cubical shape with a 10-50 nm size range. The UV-Vis absorption peaks at 387 and 415 nm suggest the formation of biogenic ZnO NPs. The EDX spectrum revealed 68.92% and 27.49% weight compositions of Zn and O, respectively, to further substantiate ZnO nanoparticle synthesis. The BC-ZnONPs showed notable anti-hyperglycaemic properties with IC50 of 87.72±5.13 and 124.21±15.20 μg/mL against α-amylase and α-glucosidase, respectively. Conclusion: Black Cumin seed extract was a useful biogenic material for synthesizing ZnO NPs. The BCZnONPs showed promising anti-hyperglycaemic properties based on the notable inhibitory activities against α-amylase and α-glucosidase enzymes. Future work may include evaluating the synergistic effects of black Cumin metabolites and ZnONPs, as well as determining the in vivo toxicity profile for safety considerations.

10aAnti-Hyperglycaemia10aBlack cumin10aZnO Nanoparticles10aα-amylase10aα-glucosidase1 aOriola, Ayodeji, Oluwabunmi1 aKar, Pallab uhttps://www.phcogj.com/article/2520