02476nas a2200181 4500008004100000245011300041210006900154260001600223300001200239490000700251520183300258653006302091100002202154700002102176700002202197700003502219856004002254 2025 eng d00aAntioxidant Activity and Tyrosinase Inhibition of Avocado (Persea americana Mill.) Leaves and Seeds Extracts0 aAntioxidant Activity and Tyrosinase Inhibition of Avocado Persea cAugust 2025 a420-4240 v173 a

Background: Ultraviolet radiation (UVR) can stimulate melanin production in the skin, leading to hyperpigmentation. One way to reduce melanin formation is by inhibiting the enzyme tyrosinase. Often discarded as waste, avocado leaves and seeds are rich in flavonoids with potential tyrosinase-inhibiting properties. Objectives: This study aimed to perform phytochemical screening, measure antioxidant and tyrosinase inhibitor activities, and explore the correlation between free radical scavenging and tyrosinase inhibition in ethanol extracts of young avocado leaves (EAL(O)) and old avocado leaves (EAL(Y)), as well as the ethyl acetate extract of avocado seeds (EAS). Methods: Antioxidant activity was assessed using the 2,2-Diphennyl-1-picrylhydrazyl (DPPH) method, while tyrosinase inhibition was evaluated in vitro with mushroom tyrosinase and L-DOPA as a substrate. Results: The results of the antioxidant activity test using the DPPH method yielded IC50 values for EAL(Y), EAL(O), and EAS of 17.9±0.15 μg/mL, 15.33±0.02 μg/ mL, and 745.66±6.08 μg/mL, respectively. Tyrosinase inhibition tests indicated that EAL(Y), EAL(O), and EAS could significantly inhibit tyrosinase activity (P <0.01), with IC50 values of 10133.26±140.12 μg/mL, 8496.20±155.06 μg/mL, and 48375.8±553.06 μg/mL, respectively. The correlation coefficients between tyrosinase inhibition and antioxidant activity for EAL(Y), EAL(O), and EAS were r = 0.955, r = 0.947, and r = 0.991, respectively. Conclusion: These findings suggest that EAL(Y), EAL(O), and EAS may be potential natural skin-whitening agents due to their flavonoid content and antioxidant and anti-tyrosinase properties.

10aPersea americana Mill; tyrosinase inhibition; DPPH; L-DOPA1 aSebayang, Shalina1 aRayendra, Raendi1 aWientarsih, Ietje1 aPriosoeryanto, Bambang, Pontjo uhttps://www.phcogj.com/article/2518