02299nas a2200253 4500008004100000245006200041210006200103260001500165300001200180490000700192520145000199653010801649100001901757700002401776700002801800700002301828700002701851700002501878700002901903700002601932700002101958700002601979856004002005 2025 eng d00aAcute and Subchronic Toxicity Study of Sampilnorov Wurile0 aAcute and Subchronic Toxicity Study of Sampilnorov Wurile cApril 2025 a203-2100 v173 a

Introduction: Sampilnorov Wurile (SW) is a traditional medicinal preparation containing 29 plant-, mineral-, and animal-derived components. It is used for cerebrovascular ischemia, retinal disorders, cardiovascular diseases, and neurological conditions. This study aimed to evaluate the acute and subchronic toxicities of SW. Methods: Acute toxicity was assessed in C57BL/6 mice based on OECD-423 guidelines, with oral doses ranging from 500 to 6,000 mg/kg body weight. Subchronic toxicity was evaluated in Wistar rats following OECD-407 guidelines, with daily oral doses of 300 and 600 mg/kg of SW daily for 4 weeks. Clinical signs, mortality, body weight, and physical condition were monitored. Hematological, biochemical, and histopathological analyses were conducted on day 29. Results: No mortality or significant clinical signs of toxicity were observed at doses up to 6,000 mg/kg, suggesting an oral median lethal dose exceeding this level. Subchronic toxicity assessment revealed no clinical signs of toxicity or mortality. SW at 300 and 600 mg/kg had no significant effects on serum biochemical parameters or vital organ histology compared to controls. Conclusion: SW exhibited no acute toxicity at doses up to 6,000 mg/kg and showed no adverse effects in a 28-d subchronic toxicity study. These findings support its safety at tested doses.

10aAcute and subchronic toxicity; biochemistry; blood test; histopathological analysis; Sampilnorov Wurile1 aChangSheng, Li1 aTserendash, Chimgee1 aGerelt-Od, Maral-Erdene1 aBatbayar, Batdavaa1 aAltankhuyag, Uyangamaa1 aBatbaatar, Mend-Amar1 aMyadagsuren, Narankhajid1 aDamdinjav, Davaadagva1 aGonchig, Enkhmaa1 aChultemsuren, Yeruult uhttps://www.phcogj.com/article/2497