02929nas a2200229 4500008004100000245014300041210006900184260001500253300001200268490000700280520219600287653001502483653001602498653001702514653001502531653002502546100001602571700002402587700002302611700002502634856004002659 2025 eng d00aMyricitrin-Mediated Biogenic Silver Nanoparticle Synthesis, Characterization, and its Antioxidant, Anticancer, and DNA Cleavage Activities0 aMyricitrinMediated Biogenic Silver Nanoparticle Synthesis Charac cApril 2025 a121-1280 v173 a

Introduction: Myricitrin (MY) is a potent antioxidant flavonoid that has recently gained research interest due to its wide applications in food, cosmetics, and medicine. Objective: The current work reports MY, its isolation and characterization from Eugenia uniflora leaves, and green synthesis with AgNO3 to afford myricitrin-based silver nanoparticles (MY-Ag NPs). Materials and Methods: The biosynthesized nanoparticles (NPs) were characterized using UV, field emission scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), High-resolution transmission electron microscopy (HRTEM) and Dynamic light scattering (DLS) methods. Antioxidant, anti-cancer, and DNA cleavage activities were based on standard in vitro bioassay methods. Results: The UV-vis absorption peak at 430 nm suggests the formation of silver-based NPs. The FESEM imaging showed spherical-to-cubical shaped MY-Ag NPs with an average size of 45.35 nm. The EDX analysis showed the presence of elemental Ag (89.40%) and N (10.22%), suggesting a successful synthesis. The XRD analysis revealed various peaks at 38.37⁰, 43.56⁰, 63.76⁰, and 77.77⁰, which suggest metallic silver reflections, further establishing the crystallinity of NPs. The MY-Ag NPs inhibited O2 -, OH-, H2O2, and NO free radicals in a dose-dependent manner. At 50 and 80 μg/mL, it demonstrated a better inhibitory effect on OH- radical than L-ascorbic acid. The cytotoxicity (IC50) against human cancer cell lines of the kidney (ACHN) and the liver (HepG2) were 54.21 ± 0.06 μg/mL and 33.36 ± 2.25 μg/mL respectively at 48 h post-treatment. Lastly, at 20 mg/mL for 120 minutes, MY-Ag NPs cleaved DNA, acting as chemical nucleases. This may suggest its capacity to impede cancer cells by cleaving the genome. Conclusion: Therefore, this study has shown that Myricitrinbased Ag NPs possess notable antioxidant and cytotoxicity that can be further exploited in the search for newer anticancer agents.

10aAnticancer10aAntioxidant10aDNA cleavage10aMyricitrin10aSilver nanoparticles1 aKar, Pallab1 aOriola, Ayodeji, O.1 aSingh, Moganavelli1 aOyedeji, Adebola, O. uhttps://www.phcogj.com/article/2487