01792nas a2200217 4500008004100000245012700041210006900168260001800237300001200255490000600267520112900273653001401402653001301416653000901429653001301438653000901451100002901460700003101489700001901520856003501539 2017 eng d00aAssessment of Biological Activity and Comparison of UPLC and RP-HPLC Chromatographic Profiles of Clausena excavata Burm.F.0 aAssessment of Biological Activity and Comparison of UPLC and RPH cFebruary 2017 a185-1910 v93 a

Clausena excavata Burm. F. (Rutaceae) has long been associated with medicinal benefits in folk medicine, particularly in the treatment of cancer and its related disorders in the eastern region of Thailand. In the the present study Ultra Pressure Liquid Chromatography (UPLC) analysis of methanolic leaf extracts of Clausena excavata were carried out using short (5 cm × 2.1 mm I.D.) columns, containing 2 μm particles at very high pressures (up to 16000 psi) to obtain high efficiency separations in very short run times. The results indicate that UPLC provided better resolution compared to routine RP-HPLC. The analysis time was drastically reduced (4 min.) which is proved to be a faster and more economical analytical tool than RP-HPLC. In addition to that, the present study also evaluates anti-fungal activity against dermatophytes Trichophyton rubrum and Trichophyton mentographytes by using an agar incorporation technique. The anti-fungal activity may be due to the presence of Angelecin and gallic acid in the plant extract.

10aAngelecin10aClausena10aHPLC10aRutaceae10aUPLC1 aChakravarthi, Guntupalli1 aReddy, Alavala, Rajasekhar1 aRamaiah, Maddi uhttp://phcogj.com/fulltext/297