02140nas a2200217 4500008004100000245010500041210006900146260001700215300000600232490000700238520153200245653000901777653000901786653000901795653001301804653002101817653001001838100001601848700001801864856004001882 2025 eng d00aEvaluation of Antioxidant Activity and Flavonoid and Phytocompounds Content in Pongamia Pinnata Seed0 aEvaluation of Antioxidant Activity and Flavonoid and Phytocompou cJanuary 2025 a60 v173 a

Introduction: Pongamia pinnata is a woody shrub and tree found in entire India mainly in dry areas. The seeds of P. pinnata are collected and traditionally oil is extracted and used to kill helminthes. The plant is a remedy for skin diseases, wound, rheumatism & ulcers and also as biofuel, green manure and fodder. This study is an attempt to know antioxidant activity of particular fraction which is having antifungal activity and flavonoid and fatty acids content which is responsible for the same. Materials and methods: In this investigation, an effort was made to know the antioxidant activity of PPEAF by DPPH assay, FRAP assay and H2O2 assay methods and Optimization of mobile phase by TLC method and supporting major flavonoid content quantification by HPTLC method Results: The result shown the ethyl acetate fraction has significant antioxidant activity of IC50 value of 212.09 (DPPH assay), 235.87 (FRAP assay) and 268.62 (H2O2 assay). The TLC method optimum mobile phase was found to be Toluene: ethyl acetate (7:3). The standard flavonoids used are Karanjin, Gallic acid and Quercetin. Conclusion: The HPTLC method results shown PPEAF was found to contain 1.465% Karanjin. GCMS result shown the PPEAF contain 13 phytocompounds in which Octadec-9-enoic acid at retention time 22.356 was found to be abundant.

10aDPPH10aFRAP10aGCMS10aKaranjin10aPongamia pinnata10aPPEAF1 aNH, Honnesh1 aSaha, Santanu uhttps://www.phcogj.com/article/2459