02741nas a2200229 4500008004100000245012000041210006900161260001800230300001400248490000700262520197800269653001502247653002402262653004002286653003402326653002902360653002402389100002102413700001602434700002102450856004002471 2024 eng d00aPhytochemical and Pharmacological Studies of Different Extracts of Stem Bark and Leaf of Flueggea leucopyrus Willd.0 aPhytochemical and Pharmacological Studies of Different Extracts  cDecember 2024 a1281-12890 v163 a

Background: Traditional medicinal plants are generally extracted and evaluated to identify potential sources of effective drugs. Objective: The present study aimed to conduct the phytochemical and pharmacological evaluation of stem bark and leaf extracts of Flueggea leucopyrus. Materials and Methods: The collected plant material was dried, powdered, and extracted separately by soxhlation with different solvents viz., petroleum ether, n-hexane, chloroform, acetone, methanol, and water. All the extracts were subjected to phytochemical evaluation. Alkaloids were extracted and characterized from the powdered sample of leaf and bark. In vitro antioxidant activity of the extracts was evaluated by DPPH (2, 2-diphenyl-1-picrylhydrazyl) and nitric oxide radical scavenging assay and anti-inflammatory activity by inhibition of protein denaturation and Human red blood cell (HRBC) membrane stabilization method. Results: Extracts were found to be either semisolid or solid with different tints of green or brown colour. The preliminary phytochemical evaluation found alkaloids, glycoside, phenolic compounds, flavonoids, carbohydrates, sterol, and saponin. On spectral evaluation, the presence of an alkaloid, Securinol-A was found in a fraction extracted from the bark. In the in vitro antioxidant and anti-inflammatory activity the tested samples showed a concentration-dependent rise of activity, particularly, the isolated fraction and acetone extract of stem bark revealed a significant activity. Conclusion: Alkaloids, flavonoids, sterols, and saponins identified in these extracts may be responsible for these biological activities. Hopefully, our in vitro and in vivo evaluations and compound-level studies in the future will reveal significant data for the development of clinically useful chemotherapeutic agents.

10aExtraction10aFlueggea leucopyrus10aIn vitro anti-inflammatory activity10aIn vitro antioxidant activity10aPhytochemical evaluation10aSpectral evaluation1 aS, Sarath, Lal P1 aM, Thirumal1 aK, Ajith, Babu T uhttps://www.phcogj.com/article/2421