02296nas a2200241 4500008004100000245011700041210006900158260001800227300001200245490000600257520154500263653003601808653002601844653002101870653002501891100001801916700001401934700001801948700001901966700001801985700001602003856003502019 2017 eng d00aHPLC Fingerprint Analysis and Content Determination of Extract with Anticancer Activities of Sedi Linearis Herba0 aHPLC Fingerprint Analysis and Content Determination of Extract w cFebruary 2017 a128-1340 v93 a

Aims: The purpose of this paper was to develop a HPLC fingerprint analysis method, compare the chromatographic fingerprints characteristics of the ethyl acetate extract with anticancer activity from Sedi Linearis Herba (Sedum lineare Thumb.) collected in different seasons and environments, determine the contents of active ingredients hyperoside, isoquercetin and astragalin of each sample by HPLC, provide believable scientific foundations for quality control and evaluation of Sedi Linearis Herba. Methods: The analyte was analyzed using BDS Hypersil C18 as chromatographic column, acetonitrile -0.1% acetic acid solution as the mobile phase (gradient elution). Results: The results showed that the major active ingredient contents of the samples collected in different seasons but at the same environment all had obvious differences. These ingredient contents of the samples collected in the end of April were much more than those in mid-August in general. Moreover, the major active ingredient contents of the samples picked in the same season but different environments were different too. For instance, the major ingredient contents of the sample in damp environment in August were more than those in sunny environment. Conclusion: All of these indicated that the HPLC fingerprint analysis and contents determination method established were very useful for quality analysis and control of Sedi Linearis Herba.

10aAnticancer Activity Ingredients10aContent Determination10aHPLC Fingerprint10aSedum lineare Thunb.1 aLiu, Diangang1 aMei, Qing1 aLong, Weifang1 aWan, Xiangluan1 aWan, Dingrong1 aWang, Luyao uhttp://phcogj.com/fulltext/289