03400nas a2200301 4500008004100000024000700041245019400048210006900242260001600311300001200327490000700339520242100346653001802767653004302785653002102828653001902849653002402868100001802892700002202910700002102932700001702953700002202970700000502992700001702997700002403014700002003038856004003058 2024 eng d a3200aPhytochemical Screening and Antibacterial Activity Test of Ethanol Extract of Durian (Durio Zibethinus murr.) Soya Varieties Against Pathogen Bacteria Escherichia Coli in Raw Drinking Water0 aPhytochemical Screening and Antibacterial Activity Test of Ethan cAugust 2024 a933-9410 v163 a

Introduction: Durian (Durio zibethinus) fruit skin contains antibacterial compounds. The antibacterial content in durian skin (Durio zibethinus) such as alkaloids, flavonoids, saponins, phenols and tannins can inhibit the growth of pathogenic bacteria such as Escherichia coli, Salmonella typhosa and Staphylococcus aureus and act as a disinfection agent. This research aims to determine the secondary metabolite compounds and antibacterial activity of the ethanol extract of Soya durian peel against the pathogenic bacteria Escherichia coli ATCC 25922. Methods: This type of research is a laboratory experiment, including making Soya durian peel extract using the maceration method using 96% ethanol solvent. The antibacterial activity test was carried out using the liquid dilution method to determine the Minimum Inhibitory Concentrations (MIC) value and the solid dilution method to determine the Minimum Bactericidal Concentration (MBC) value. Results: Based on qualitative phytochemical screening, Soya durian peel ethanol extract contains secondary metabolite compounds with an average content of 4.24% alkaloids, 22.95% flavonoids, 1.74% saponins, 57.41% phenols and 2.27% tannins. Soya durian peel extract has an MIC against E. coli ATCC 25922 bacteria of 3.12%, while the MBC value of Soya durian peel extract against E. coli ATCC 25922 bacteria is 6.25%. The results of the One Way ANOVA analysis of the Minimum Bactericidal Concentration (MBC) data have a significant value of 0.00 < 0.05. The results of the Pearson correlation test (r) showed a significant number of 0.000 (p < 0.05), the Pearson correlation coefficient between concentration and number of bacterial colonies was (r) = 0.812. This means that the higher the concentration of Soya durian peel ethanol extract given, the less the number of E.coli ATCC 2592 bacterial colonies will be reduced. The results of a simple linear regression test showed that the value of Y = 245.618 – 29.016 245,618 colonies and each increase in the concentration of Soya durian peel ethanol extract by 1% will cause a decrease in the number of bacterial colonies to 29,016 colonies. Conclusion: Soya durian skin extract has antibacterial compounds that can kill pathogenic Escherichia coli bacteria in raw drinking water.

10aAntibacterial10aDurian (Durio zibethinus) Soya variety10aEscherichia coli10aPhytochemicals10araw drinking water.1 aAhmad, Rahwan1 aAmiruddin, Ridwan1 aArsin, A.Arsunan1 aStang, Stang1 aIshak, Hasanuddin1 a1 aAlam, Gemini1 aWispriyono, Bambang1 aMallongi, Anwar uhttps://www.phcogj.com/article/2361