02657nas a2200229 4500008004100000245011900041210006900160260001400229300001200243490000700255520195300262653002102215653001502236653001002251653001302261653001502274100002802289700003202317700001802349700002002367856004002387 2024 eng d00aCorrelation of N-Cadherin and MMP-9 Expression with Regional Nodal Metastasis in Laryngeal Squamous Cell Carcinoma0 aCorrelation of NCadherin and MMP9 Expression with Regional Nodal cJune 2024 a679-6830 v163 a

Background: In laryngeal carcinoma, regional nodal metastasis serves as a significant prognostic factor. A special biomarker is needed to predict the status of nodal metastases. N-cadherin, a binding protein, aids in cell migration and enabling tumor cells to spread to new locations. The involvement of matrix metalloproteinase-9 (MMP-9) in metastasis includes fostering the dissemination of tumor cells from the primary tumor and enhancing tumor cell motility. Objective: This study seeks to explore the distinctions and relationships in N-cadherin and MMP-9 expression among patients with laryngeal squamous cell carcinoma at various N stages. Methods: Employing a cross-sectional approach, this study conducted an analytic observational investigation on formalin-fixed paraffin-embedded patients whose histopathological diagnosis is laryngeal squamous cell carcinoma in the Pathology Laboratory of Dr. Soetomo Regional Public Hospital in 2018–2021. The samples were divided into N stages based on radiological imaging from the CT scan. Immunohistochemistry examinations were performed using N-cadherin and MMP-9 antibodies and scored using the immunoreactive score (IRS), based on percentage and intensity. The differences in expression and correlation between N-cadherin and MMP-9 were analyzed using statistical tests. Results: Statistical insignificance was observed in N-cadherin expression at various N stages (p = 0.099). There were significant differences in MMP-9 expressions at various N stages (p = 0.0006338). There was no correlation between N-cadherin and MMP-9 expression at various N stages in laryngeal squamous cell carcinoma (p = 0.0638, rs = 0.27). Conclusion: In laryngeal squamous cell carcinoma, MMP-9 serves as a predictor for lymph node metastasis, which, if present, deteriorates the patient's prognosis.

10aLaryngeal cancer10aMetastasis10aMMP-910aN stages10aN-cadherin1 aKusumastuti, Etty, Hary1 aDjuanda, Stephanie, Natasha1 aAriani, Grace1 aMastutik, Gondo uhttps://www.phcogj.com/article/2317