02262nas a2200217 4500008004100000245007700041210007000118260001500188300001200203490000700215520161800222653003501840653003101875653001101906653001201917653000901929100002801938700001801966700002001984856004002004 2024 eng d00aHIF-1α and VEGF Expression in Adult-type Diffuse High-Grade Astrocytoma0 aHIF1α and VEGF Expression in Adulttype Diffuse HighGrade Astrocy cApril 2024 a466-4700 v163 a

Background: Gliomas stand as the prevalent primary malignant brain tumors in adults with astrocytoma being more common than oligodendroglioma. Based on isocitrate dehydrogenase (IDH) status, astrocytomas are classified as astrocytoma with mutated IDH and astrocytoma with wild-type IDH (glioblastoma). Tumor growth relies on angiogenesis, a process facilitated by key factors such as Vascular Endothelial Growth Factor (VEGF) and Hypoxia Inducible Factor-1α (HIF-1α). This study aims to investigate the VEGF and HIF-1α expression profiles in grade 4 astrocytomas, encompassing both mutated IDH and wild-type IDH. Method: This study was conducted on 43 formalin fixed paraffin embedded (FFPE) materials of surgical specimens from adult-type grade 4 astrocytoma. Immunohistochemistry with IDH1 R132H was carried out to determine the IDH status, followed by assessment of HIF-1α and VEGF expression using semi-quantitatively utilizing immunoreactive score (IRS), and categorized as negative, weak, moderate, and strong. Results: Statistical analysis revealed no disparity in HIF-1α expression between both tumor types, nor was there a difference in VEGF expression in both tumor types, yet a positive association was established between VEGF and HIF-1α expression levels in IDH mutant and wild type of grade 4 astrocytoma with moderate strength (r=0.433). Conclusion: HIF-1α and VEGF are positively linked, despite the IDH status, and simultaneously work to promote angiogenesis in diffuse high-grade astrocytoma.

10aAstrocytoma IDH mutant grade 410aGlioblastoma IDH wild type10aGlioma10aHIF-1α10aVEGF1 aPatikawa, Febria, Rizky1 aFauziah, Dyah1 aSandhika, Willy uhttps://www.phcogj.com/article/2275