02649nas a2200229 4500008004100000245014500041210006900186260001800255300001400273490000700287520189700294653002002191653001802211653001602229653001902245100002902264700002402293700001902317700002002336700002302356856004002379 2023 eng d00aPerformance Comparison of Urine Sediment Analytical Tool by Flowcytometry and Digital Imaging with Standardized Manual Microscopic Testing0 aPerformance Comparison of Urine Sediment Analytical Tool by Flow cDecember 2023 a1189-11960 v153 a

Background: Urine sediment examination provides numerous information about patient's kidney condition. Flowcytometry and digital imaging system could conduct automatic urine sediment analysis. Therefore, determining the diagnostic performance of the examination is very important in the management carried out by the clinician. This study aims to determine the diagnostic performance of urine flowcytometry and digital imaging sediment examination tools compared to manual microscopes that are standardized as gold standards. Methods: This study is an analytical observational study with a cross-sectional approach on 92 urine samples of patients who attended the Internal Medicine Nephrology Polyclinic of Dr. Soetomo Academic Hospital Surabaya. The performance of flowcytometry and digital imaging methods is assessed by calculating sensitivity and specificity. Analysis of the suitability of each urine sediment parameter used Cohen's kappa. Urine sediment analysis with a light microscope was conducted with the Shih-Yung method as a gold standard. Results: The erythrocyte parameters revealed a very favorable result with the concordance of the flowcytometry with the Shih-Yung method (κ=0.82) and fair results for WBC (κ=0.25), Epithelium (κ=0.57) and Cast (κ=0.27). At the same time, yeast had substantial conformity (κ=0.63). The digital imaging method showed substantive fit for WBC (κ=0.676), RBC (κ=0.621), fair for SEC (κ=0.42) and NSE (κ=0.24), moderate for Yeast (κ=0.45), and slight for Hyaline Cast (κ=0.074) and Path Cast (κ= 0.134) Conclusion: The urine flowcytometry demonstrates better performance compatibility with a standardized manual microscope compared to urine digital imaging. However, pathological samples should still be verified with a manual microscope

10aDigital imaging10aFlowcytometry10aMicroscopic10aUrine sediment1 aRitonga, Nur, Fitri Haya1 aMarpaung, Ferdy, R.1 aKahar, Hartono1 aMardiana, Nunuk1 aPuspitasari, Yessy uhttps://www.phcogj.com/article/2184