01982nas a2200205 4500008004100000245018100041210006900222260001800291300001400309490000700323520125300330653004801583653002201631100002201653700000501675700002301680700000501703700002801708856004001736 2023 eng d00aComparison of the Anti-Inflammatory Activity of Flavonoid Bioactive Compounds Acetone Fraction and Steroid Fraction Ethyl Acetate Sungkai Leaves In Vivo and In Silico Studies0 aComparison of the AntiInflammatory Activity of Flavonoid Bioacti cDecember 2023 a1068-10760 v153 a

One of the medicinal plants that is widely used by traditional communities is sungkai (Peronema canescens Jack), which is known to contain secondary metabolite compounds of flavonoids, saponins, tannins, steroids, terpenoids, alkaloids, and phenols. This research aims to isolate secondary metabolite compounds from acetone and ethyl acetate fractions, which act as anti-inflammatory agents in vivo and in silico. Acetone and ethyl acetate fractions were isolated and characterized using UV-Vis and FT-IR. The In Vivo test was carried out on Mus musculus, while the In Silico test was carried out using the IL-10 and IFN-γ receptors, which play a role in inflammation and immunity. Our research results showed that the ethanolic (F1) and n-hexane (F2) fractions of P. canescens extract had good anti-inflammatory activity with percent inhibition values of 58.12% and 56.59%, respectively. The characterization results showed that isolate F1 was a flavonoid group, Naringenin compound, while isolate F2 was a steroid group, β-Sitosterol compound. Moreover, from the scoring docking results, β-Sitesterol has tremendous potential as an anti-inflammatory than the compound naringenin.

10aAnti-inflamatory; β-Sitesterol; Naringenin10aP. canescens Jack1 aLatief, Madyawati1 a1 aFahrezi, Arliangga1 a1 aTarigan, Indra, Lasmana uhttps://www.phcogj.com/article/2164