02859nas a2200301 4500008004100000024000700041245011100048210006900159260001700228300001200245490000700257520192700264653001102191653002802202653003702230653002302267653001202290653001702302100002502319700002802344700003002372700001602402700002902418700001702447700002302464700003002487856004002517 2023 eng d a3600aEffect of Lactobacillus Plantarum IS-10506 on Paneth Cell Regeneration in the Ileum of Sprague Dawley Rats0 aEffect of Lactobacillus Plantarum IS10506 on Paneth Cell Regener cOctober 2023 a928-9320 v153 a

Background: Pathogenic Escherichia coli (E. coli) is the most common infectious agent among children in developing countries. Indigenous probiotics are not widely used to treat diarrhea and intestinal infections. This study aims to investigate the cell regeneration process of paneth cells after administration of Lactobacillus plantarum IS-10506 due to damage caused by Lipopolysaccharide (LPS) E. coli O55:B5, through the expression of MATH-1 and DEFA-6. Methods: This study used 64 paraffin blocks from Rattus norvegicus strain Sprague-Dawley divided into four groups. There were three treatments, KN, KL, KP and KPR groups, The KN group represent the administration of placebo. The KL group received LPS E. coli O55:B5 on day one. The KP group received LPS E. coli O55:B5 on the first day and Lactobacillus plantarum IS-10506 on the second day until six-day. The KPR group were administered Lactobacillus plantarum IS- 10506 six days prior to receiving LPS E. coli O55:B5, respectively. All groups, except KN, received LPS at a dose of 250 μg/kg body weight once, and Lactobacillus plantarum IS-10506 at a dose of 2.86x1010 CFU/ day. Evaluating paneth cell regeneration, DEFA-6, and MATH-1 expression immunohistochemistry was conducted on all tissues. Results: The expression of DEFA-6 and MATH-1 in the KP and KPR groups on day three of observation was significantly higher from the KL group. Even though the KL group achieved significant growth, the results of this expansion were significantly smaller than KP and KPR groups. Conclusion: After mucosal injury caused by LPS E. coli O55:B5, administration of probiotic Lactobacillus plantarum IS-10506 may increase paneth cell regeneration through differentiation and cell number.

10aDEFA-610aEscherichia coli O55:B510aLactobacillus plantarum IS-1050610aLipopolysaccharide10aMATH-1.10aPaneth cells1 aHutama, Rasio, Putra1 aAthiyyah, Alpha, Fardah1 aRanuh, I.G.M., Reza Gunad1 aDarma, Andy1 aSumitro, Khadijah, Rizky1 aRiawan, Wibi1 aSurono, Ingrid, S.1 aSudarmo, Subijanto, Marto uhttps://www.phcogj.com/article/2144