02135nas a2200229 4500008004100000024000700041245006800048210006800116260001700184300001200201490000700213520149000220653002101710653002301731653001901754653002201773100001701795700002201812700002601834700000501860856004001865 2023 eng d a3500aCardioprotective Effects of Thymoquinone on Myocardial Fibrosis0 aCardioprotective Effects of Thymoquinone on Myocardial Fibrosis cOctober 2023 a924-9270 v153 a

Introduction: Thymoquinone (TQ) is one of the active ingredients in herbal plants such as Nigella sativa which has antioxidant and anti-inflammatory properties thus may inhibits cardiac fibrosis formation. This study aims to determine the effectiveness of Thymoquinone as a cardioprotective agent in suppressing the extent of fibrosis in Wistar rats induced with lipopolysaccharide (LPS). Methods: This post-test only control study used 30 Wistar rats which were divided into 5 groups: saline, LPS-induced cardiac fibrosis, LPS-induced cardiac fibrosis treated with TQ 10 mg/mL, LPS-induced cardiac fibrosis treated with TQ 20 mg/mL, and LPS-induced cardiac fibrosis treated with TQ 40 mg/mL. Serum IL-6, GSH, and cTnT levels were measured using ELISA, and Mason's trichrome staining was used to assess myocardial fibrosis. Results: The LPS10+TQ20 and LPS10+TQ40 groups exhibited significantly lower levels of IL-6 compared to the LPS10+TQ10 group (p < 0.05). GSH levels did not show a significant decrease in the TQ groups across different doses (p=0.771). The TQ-treated group demonstrated lower cTnT levels compared to the LPS-only group (p<0.05). Thymoquinone treatment resulted in reduced fibrosis area compared to the LPS10 group (p<0.05). Conclusions: TQ has a promising cardioprotective effect on the formation of cardiac fibrosis in Wistar rats induced with LPS.

10aCardiac Fibrosis10aLipopolysaccharide10aNigella sativa10aOxidative stress.1 aAbduh, Saugi1 aBambang, Purwanto1 aParamasari, Dirgahayu1 a uhttps://www.phcogj.com/article/2143