02938nas a2200277 4500008004100000024000700041245016400048210006900212260001700281300001200298490000700310520205800317653001402375653001302389653002202402653002102424653001502445100002702460700002902487700002102516700002502537700001602562700001602578700002602594856004002620 2023 eng d a1600aEvaluation of In vivo Analgesic and Anti-inflammatory Activity of Oroxyulum indicum, Baicalein, Chrysin with Phytochemical Analysis and Molecular Docking Study0 aEvaluation of In vivo Analgesic and Antiinflammatory Activity of cOctober 2023 a811-8220 v153 a

Background: Oroxyulum indicum (OIE) is a native medicinal plant that has been widely employed in Ayurvedic medicine for thousands of years. Though studies have been published citing the analgesic and anti-inflammatory activity of Oroxyulum indicum and chrysin and Baicalein, there has been no comparative study comparing their activittes and confirming them with molecular docking results. Molecular docking study of two phytochemicals Chrysin (PubChem CID 5281607) and Baicalein (PubChem CID 5281605) into the active sites of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2). Evaluation and validation of Anti-inflammatory and Analgesic effects of a methanolic extract of the stem bark of Oroxylum indicum and its two constituents Chrysin and Baicalein in Charles foster rats with analysis of the phytoconstituent of Oroxyulum indicum through HRMS analysis. Methodology: UHPLC-HRMS/MS analyses were performed on a Dionex Ultimate 3000 RS Series UHPLC system combined with a Q Exactive Plus High-Resolution Accurate Mass Spectrometry System. Hot plate and Tal flick model are used for screening of analgesic activity. TNF-alpha and IL-6 inflammatory markers were examined. Carrageenan model is used for antiinflammatory analysis. Result: Interesting results has been obtained in the docking studies of Chrysin and Baicalein with COX-1 (PDB ID: 1EQG). The hydrogen bond interaction established between the Chrysin and Baicalein with the important amino acid, includes Arg 120, Tyr 355, Ser 530, Met 522 (Figure 1). The binding free energy of the Chrysin and Baicalein with target COX-1 was found to be -7.88 and -7.26 Kcal/mol. Conclusion: There is marked reduction in the TNF Alpha expression in the OIE group which is followed by Baicalein and Chrysine. The Baicalein group shows the most marked cumulative increase in reaction time for tail flick among all the groups of the intervention group followed by Chrysine and OIE.

10aBaicalein10aChrysine10aMolecular docking10aOroxylum indicum10aTNF alpha.1 aPathak, Bhairav, Kumar1 aPalandurkar, Kamlesh, M.1 aSingh, Meenakshi1 aTrigunayat, Anshuman1 aSingh, Amit1 aGiri, Reena1 aGiri, Kiran, Rajendra uhttps://www.phcogj.com/article/2123