02703nas a2200289 4500008004100000024000700041245018200048210006900230260001700299300001200316490000700328520177900335653001202114653002802126653000902154653002102163100001902184700001902203700002302222700001902245700001802264700002102282700003102303700001802334700002102352856004002373 2023 eng d a0100aDecreased Expression of Endocrine Glands Vascular Endothelial Growth Factor (EG-VEGF) in Rat Endometrial After Stimulation with Recombinants FSH Can be Reduce Implantation Rates0 aDecreased Expression of Endocrine Glands Vascular Endothelial Gr cOctober 2023 a707-7130 v153 a

Background: Decreased expression of EG-VEGF in human endometrium after administration of ovarian stimulators has been reported to adversely affect endometrial receptivity and low implantation rates. Objective: To determine the effect of recombinant FSH administration on EG-VEGF protein in the endometrium of rats taken in the secretory phase and how it relates to endometrial receptivity. Design: A total of 36 female wistar rats with normal estrous cycles were randomly assigned to the natural cycle group (NC) and two stimulation groups (SC) which were injected with recombinant FSH at 12.5 IU and 25 IU intraperitoneally. Uterine necropsy and blood collection were performed on day 1, day 2, and day 3 after hCG administration. A total of 3 female rats from each group were mated with male rats (two males and three females in one cage). A successful marriage is indicated by the presence of a vaginal plug the next day. The level of EG-VEGF protein expression was assessed by immunohistochemical technique and steroid hormone levels were measured by the Elisa technique. Results: ANOVA test, that the expression of EG-VEGF in the endometrial glands showed a significant decrease from the normal cycle group to the stimulated cycle group 1 (SC 1) and SC2 (P = 0.00), as well as the expression of EG-VEGF in the endometrial stroma. (P = 000). Steroid hormone levels did not show a significant decrease between the normal cycle group and the stimulated cycle group (P = 0.48 and P = 0.13). Conclusion: Decreased EG-VEGF expression in rat endometrium after administration of recombinant FSH is associated with decreased endometrial receptivity which can reduce pregnancy rates.

10aEG-VEGF10aEndometrial receptivity10arFSH10aSecretory phase.1 aMuharam, Raden1 aSahar, Nurhuda1 aKusmardi, Kusmardi1 aYunaini, Luluk1 aNovita, Risqa1 aThuffi, Rosalina1 aTjampakasari, Conny, Riana1 aBirowo, Ponco1 aKristanty, Diyah uhttps://www.phcogj.com/article/2108