02582nas a2200229 4500008004100000245009000041210006900131260001900200300001200219490000600231520186800237653001502105653001202120653001902132653003702151100002602188700002802214700001802242700003402260700001902294856003902313 2016 eng d00aGc-Ms Analysis of Ethanolic Stem Extract of Clausena anisata (Willd.) Hook F Ex Benth0 aGcMs Analysis of Ethanolic Stem Extract of Clausena anisata Will cSeptember 2016 a576-5780 v83 a

Objective: In the present study, the bioactive components present in the ethanol stem extract of Clausena anisata was analyzed by using Gas Chromatography Mass Spectrometry analysis technique (GC-MS). Clausena anisata, a medicinal plant belonging to the family Rutaceae, is represented by 20 species available in India and used traditionally for the treatment of several ailments but there is a requirement to identify its phytoconstituents, its target, mode of action and treatment using plant products either alone or in combination with synthetic drugs. Methods: Clausena anisata stem was procured from Manamettupatti, a village of Pudukottai District, Tamil Nadu. The shade dried stem was powdered and extracted using ethanol by maceration method. One microlitre of the extract was subjected to GC-MS analysis to detect the presence of bioactive compounds present in the stem of C. anisata. Results:The results showed that the ethanol stem extract of C. anisata contained nine bioactive compounds, of which the major one is n-hexadecanoic acid (78.54%), followed by 8-octadecenoic acid, methyl ester, [E]- (6.638%). The total number of compounds obtained was compared with National Institutes of Standard and Technology (NIST) library that contains more than 62,000 known compounds based on retention time and molecular mass. Conclusion: In this study, nearly nine compounds have been identified from the ethanolic stem extract of C. anisata using GC-MS analysis which was mainly composed of fatty acids and sterols. The GC-MS analysis is used to understand the nature of active principles present in this plant revealed that the plant can be used as a potential source of new useful drugs.

10aC. anisata10aEthanol10aGC-MS analysis10amaceration and phytoconstituents1 aYakoob, Arsia, Tarnam1 aTajuddin, Nargis, Begum1 aMathew, Shilu1 aHussain, Muhammad, Ilyas Moha1 aQadri, Ishtiaq uhttps://www.phcogj.com/article/210