01981nas a2200241 4500008004100000024000700041245010000048210006900148260001600217300001200233490000700245520126500252653001201517653001601529653001501545653001301560653001101573100003101584700003201615700003201647700002001679856004001699 2023 eng d a2300aHPLC Isolation of Rutin, Hesperidin and Quercetin from Ruta Chalepensis Extract Growing in Iraq0 aHPLC Isolation of Rutin Hesperidin and Quercetin from Ruta Chale cAugust 2023 a606-6110 v153 a

Background: Ethnopharmacological relevance: Ruta chalepensis L For years, various portions of this plant have been utilized in traditional medicine to treat a number of illnesses, including nervous disorders, rheumatism, menstrual issues, fever, convulsions, mental disorders, dropsy, and neuralgia. Objection: In this study, we aim to characterize three flavonoids extracted from Ruta chalepensis L. Material and Methods: In this study, various concentrations (80% and 100%) of methanol and ethanol in water were used as solvents in the extraction of flavonoids (rutin, hesperidin, and quercetin) from Ruta chalepensis L. by using conventional extraction methods (reflex and maceration). Result: HPLC results confirm Using maceration with 80% methanol was recommended for extracting flavonoids from Ruta chalepensis L. to obtain the highest yields, whereas reflex with 80% ethanol showed a slight increase in quercetin amount. The phytochemical screening of Ruta chalepensis L. exhibited the presence of flavonoids. Conclusion: The highest amount of the three flavonoids was found in maceration with 80% methanol.

10aEthanol10aExtraction.10aMaceration10aMethanol10aReflex1 aAl-Jaberi, Ali, Mohammed Z1 aAl-Fadal, Sabaa, Ali Mohamm1 aAbdul-Jalil, Thukaa, Zuhair1 aAl-Wafi, Haider uhttps://www.phcogj.com/article/2088