02621nas a2200217 4500008004100000024000700041245016800048210006900216260001500285300001200300490000700312520187100319653002602190653001802216653002002234653005102254653001602305100002602321700001602347856004002363 2023 eng d a1900aHPTLC Comparision Studies with Marker Compound and Isolation of Rutin Anti Inflammatory Agent from Ethanolic Leaf Extract of Rivea Hypocrateriformis (Desr.) Choisy0 aHPTLC Comparision Studies with Marker Compound and Isolation of  cApril 2023 a353-3580 v153 a

Introduction: To extract the leafy parts with ethanol and perform the HPTLC studies with marker compounds Quercetin, Rutin, and gallic acid then a compound was isolated and susceptible to spectroscopic analysis Background: Rivea hypocrateriformis (Desr.) Choisy holds Convolvulaceae family the leafy parts underwent a Soxhlet extraction with ethanol which was then subjected to a phytochemical investigation that shows the presence of alkaloids, phenols, flavonoids, terpenoids, tannins, glycosides saponins, sterols, and carbohydrates. Material and Methods: Firstly, the ethanolic leaf extract is treated with petroleum ether to remove excess lipids, sterols, and impurities. TLC was performed by toluene: ethyl acetate: formic acid and the same solvent system was used for column chromatography. The bands getting separated into the column were collected separately in a test tube. Once the solvent toluene ends, the next solvent system with 18 ml of toluene and 2 ml of ethyl acetate, is introduced into the column, and continues the separation procedure. Collect separated bands and perform TLC of each separate band by using the same solvent system in a concentration of 5:4:1. A single component on a TLC plate with a solvent system of 12 ml toluene and 8 ml ethyl acetate was obtained whose characterization was done by IR, NMR, and mass. Results: The RF value of Rutin is 0.17 which correlates with the Retention factor of ethanolic leaf extracts of RH by HPTLC comparative studies and a compound was isolated by column chromatography. Conclusion: These studies have concluded that a compound is separated by means of HPTLC and spectroscopic interpretation and identified as Rutin.

10aColumn chromatography10aHPTLC studies10aMarker compound10aRivea hypocrateriformis ethanolic leaf extract10aTLC plates.1 aG, Shanthini, Nachiar1 aM, Thirumal uhttps://www.phcogj.com/article/2008