02029nas a2200193 4500008004100000024000700041245011500048210006900163260001500232300001200247490000700259520132400266653011401590100002101704700002601725700002101751700002301772856004001795 2023 eng d a3200aInfluence of the Environment on Biofilm Formation Candida albicans of Vulvovaginal Candidiasis Isolate Patient0 aInfluence of the Environment on Biofilm Formation Candida albica cMarch 2023 a216-2220 v153 a

Context: Candida albicans is a type of fungus that can produce biofilms and may cause Vulvovaginal Candidiasis (VVC) disease. We investigated the effect of environment on biofilm formation of C. albicans patient isolates and ATCC 14053. Biofilm formation is influenced by several factors such as environments and nutrients. Objectives: To investigated the effect of environment on biofilm formation of C. albicans patient isolates and ATCC 14053. Methods: The samples using C. albicans ATCC 14053, C. albicans, which may form biofilms, was isolated from patient Dermatology and Venereology and Obstetrics and Gynecology from a hospital in Malang. TCP (A tissue Culture Plate) is the biofilm formation method used. Results: Biofilm formation took 48-72 hours at 25 °C and 96-120 hours at 37 °C. Based on the result biofilm formation of C. albicans is influenced by environmental factors and characterized by a high OD value. Conclusions: Biofilm formation is accelerated in temperature incubation needed at 25 °C for 48-72 hours, using biomass 107 CFU/mL, nutrition using Potato Dextrose Broth media and 1% glucose, and the solvent of 30% acetic acid to obtain acid condition.

10aBiofilm formation; Candida albicans; sabourau dextrose broth; potato dextrose broth; vulvovaginal candidiasis1 aAnggraini, Wirda1 aPurwanto, Djoko, Agus1 aKusumawati, Idha1 aIsnaeni3, Suryanto uhttps://www.phcogj.com/article/1984