01989nas a2200229 4500008004100000024000700041245006600048210006500114260001700179300001400196490000700210520132500217653001801542653002201560653002701582653002601609653003101635100001701666700001701683700001901700856004001719 2022 eng d a2400aAntibacterial Effect of Nigella sativa L. Seed from Indonesia0 aAntibacterial Effect of Nigella sativa L Seed from Indonesia cJanuary 2023 a1029-10320 v143 a

Nigella sativa L. was allegedly to has antibacterial effect. The aim of this research is to investigate antibacterial effect of ethanolic extracts of 96% Nigella sativa L. from Indonesia toward Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus epidermidis. The Antibacterial activity of of Nigella sativa L toward Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus epidermidis using the well method. The 96% ethanolic extract of 12,5; 25;50 and 100% (mg/ml)inhibit growth of Staphylococcus aureus (with inhibition zone 11.06; 29.58; 28.22; 30.84 mm respectively) and Streptococcus epidermidis (20.90; 31.90; 29.93; 33.07 mm respectively). Ethyl acetate fraction of 96% ethanolic extract of Nigella sativa concentration of 6,25; 12,5; 25;50 and 100% (mg/ml) inhibit growth of Staphylococcus aureus (12.91; 15.06; 19.19; 37.48; 46.18 mm respectively and Streptococcus epidermidis (19.07; 19.21; 20.22; 21.62; 40.00 mm respectively). Ethanolic 96% extract and ethyl acetate fraction of ethanolic extract of Nigella sativa have antibacterial effect toward Staphylococcus aureus and Streptococcus epidermidis invitro.

10aAntibacterial10aNigella sativa L.10aPseudomonas aeruginosa10aStaphylococcus aureus10aStreptococcus epidermidis.1 aSutrisna, Em1 aWahyuni, Sri1 aFitriani, Aris uhttps://www.phcogj.com/article/1945