02742nas a2200241 4500008004100000245015500041210006900196260001300265300001200278490000600290520191500296653002502211653001902236653003002255653002202285653001902307653003102326100002702357700002202384700002902406700002602435856003902461 2016 eng d00aGas Chromatography - Mass Spectrometry Analysis and In vitro Antioxidant Activity of the Ethanolic Extract of the Leaves of Tabernaemontana divaricata0 aGas Chromatography Mass Spectrometry Analysis and In vitro Antio cOct 2016 a451-4580 v83 a

Objective: To identify phytoconstituents present in an ethanolic extract of the leaves of Tabernaemontana divaricata and to evaluate its in-vitro antioxidant potential. Methods: The extract was subjected to gas chromatography- mass spectrometry analysis to identify phytoconstituents, and screened for hydroxyl, superoxide and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and metal-chelating activity as a measure of potential antioxidant activity. Results: GC-MS analysis of the extract revealed the presence of 96 phytoconstituents, of which 17 are reported to be bioactive and 11 of these to possess antioxidant potential. When tested in-vitro, the extract exhibited the most potent radical-scavenging activity at a maximum concentration of 10 mg/ml, scavenging effects of 64%, 67% and 69% and corresponding half maximal inhibitory concentration (IC50) values of 6.7 mg/ml, 6.8 mg/ml and 6.2 mg/ml on hydroxyl, superoxide and DPPH radicals, respectively. Ascorbic acid used as a standard (10 mg/ml) showed scavenging effects of 73%, 73% and 75% and corresponding IC50 values of 5.3 mg/ml, 5.8 mg/ml and 5.2 mg/ml, respectively, on hydroxyl, superoxide and DPPH radicals. At 10 mg/ml, the extract and an ethylenediaminetetraacetic acid standard exhibited 68% and 78%, respectively, chelation of ferrous ions; at the same concentration, the reducing power of the extract and that of a butylated hydroxytoluene standard was found to be 3.855 and 4.308, respectively. Conclusion: These observations strongly suggest that the ethanolic extract of T. divaricata leaves has potent in-vitro antioxidant activity and thereby could act as a possible therapeutic agent for oxidative stressinduced pathological states.

10aantioxidant activity10aGC-MS analysis10aMetal chelating activity.10aPhytoconstituents10aReducing power10aTabernaemontana divaricata1 aAnbukkarasi, Muniyandi1 aThomas, Philip, A1 aSundararajan, Mahalingam1 aGeraldine, Pitchairaj uhttps://www.phcogj.com/article/190