02185nas a2200241 4500008004100000024000700041245011900048210006900167260001700236300001200253490000700265520147000272653001001742653000801752653000801760653001201768100002101780700002101801700002301822700003001845700002801875856004001903 2022 eng d a2400aStudy of Sericin Sequences from Bombyx mori as Antiaging through ROS with Molecular Simulation and DPPH Evaluation0 aStudy of Sericin Sequences from Bombyx mori as Antiaging through cOctober 2022 a632-6410 v143 a

The presence of ROS is associated with aging, which is damage caused by free radical reactions. ROS causes oxidation of low density lipoprotein (LDL), which builds up in plaque and contributes to inflammation. With aldehyde secondary products of lipid peroxidation such as Malondialdehyde (MDA), lipoxygenase, and xanthine oxidase as markers of oxidative stress, oxidized LDL causes endothelial dysfunction and cell apoptosis. The antioxidant 1,1 diphenyl-2-picrylhydrazyl (DPPH) sericin from Bombyx mori was tested in silico and in vitro in this study. The Bombyx mori peptide sequences QAYADYHSDPNGGSA (SP4) and ASSSFDASSA (SP7) had lower Gibbs energy for lipooxygenase (LOX) than native ligands, with values of -23.1044, -21.0056, and -10.3275 kcal/mol, respectively. hydrogen bonding to Gln289, Asp293, and Gly569. While ASSSFDASSA (SP7) has a higher Gibbs energy for xanthine oxidase (XOX), SEASSSTQATTVS (SP 5) has a lower Gibbs energy with values of -20.1839, -17.8952, and -11.8921 kcal/mol, respectively. While the cavity binding of the xanthine oxidase peptide binding SP5 and SP7 is located at the Glu802, Asp872, and Ser876 binding sites, the DPPH test confirmed in vitro that the 10% sericin Gel had an IC50 of 19.7394 ppm compared to 3.71 ppm ascorbic acid. The findings of the preceding study demonstrate that sericin, as an antioxidant, is one of the candidates for antiaging.

10aDPPH.10aLOX10aROS10aSericin1 aAgustina, Fitria1 aFadilah, Fadilah1 aPangkahila, Wimpie1 aWiraguna, Anak, Agung Gde1 aDewi, Gusti, Ayu Sri Ma uhttps://www.phcogj.com/article/1881