02067nas a2200229 4500008004100000024000700041245010500048210006900153260001700222300001200239490000700251520137600258653001501634653001201649653001401661653001601675100002701691700001901718700003301737700002701770856004001797 2022 eng d a0800aPotential Role of Mitragynine as Lipolysis Stimulator via Adrenergic Signalling: Docking Model Study0 aPotential Role of Mitragynine as Lipolysis Stimulator via Adrene cOctober 2022 a527-5310 v143 a

Backgrounds: Mitragynine is the most popular of the more than 50 alkaloids contained in M.Speciosa. In particular, the Mitragynine alkaloid has the potential to increase lipid (fats) metabolism through specific pathways such as adenylyl cyclase signaling via adrenergic receptors. In this case, Asp Amino acid and Ser are the types of residues that can activate adenylyl cyclase to initiate a series of activities in cells. Methods: This study used Mitragynine ligand and adrenergic receptors (α1b, α2a, α2b, α2c dan β1). The receptor candidates were tested using Autodock whose test results were presented in the form of tables and 3-dimensional images using the Biovia Discovery Studio. Results: Hydrogen bonds were formed between Mitragynine and the amino acids Asp and Ser at the β1-adrenergic receptor. The binding amino acids were found in Ser20 and Asp21 with energy bond of -5.26 kcal/mol and IC50: 111.35 ppm. Meanwhile, at the adrenergic receptor α2b there was only Asp residue that formed hydrogen bond with Mitragynine namely Asp218A. The energy bond formed between the two was -5.19 kcal/mol and IC50: 125.04 ppm. Conclusion: Mitragynine has the potential to stimulate lipolysis through the pathways of α2b and β1-adrenergic receptors.

10aAdrenergic10aDocking10aLipolysis10aMitragynine1 aAbidin, Khoirul, Rista1 aLesmana, Ronny1 aSyamsunarno, Mas, Rizky Angg1 aDharma, Kelana, Kusuma uhttps://www.phcogj.com/article/1865