02490nas a2200229 4500008004100000245016200041210006900203260001600272300001200288490000700300520171500307653001602022653001402038653002402052653001502076100002902091700002402120700002602144700002902170700002102199856004002220 2022 eng d00aQuantification of total polyphenols and flavonoids, antioxidant activity, and Sinensetin and Imperatorin contents of Imperata cylindrica root ethanol extract0 aQuantification of total polyphenols and flavonoids antioxidant a cAugust 2022 a327-3370 v143 a

Introduction: Imperata cylindrica, commonly known as cogon grass, is currently widely distributed and used as a medicinal plant. The major compounds that have been isolated and identified are polyphenols and flavonoids, which have many biological activities such as antioxidant, and anticancer. Polyphenols and flavonoids are mostly found in the roots and leaves. This study aimed to perform phytochemical screening on I. cylindrica root ethanol extract from Sragen, Central Java, Indonesia and determine the total polyphenol, flavonoid, antioxidant activity and quantify Sinensetin and Imperatorin contents of the extract. Method: Quantification of all parameters were measured using visible spectrophotometric methods. Total polyphenol, total flavonoid contents, as well as antioxidant activity were measured using Folin-Ciocalteu reagent, aluminum chloride reagent, and 1,1-diphenyl-2-picrylhydrazyl, respectively, and quantification of Sinensetin and Imperatorin were measured using High Performance Liquid Chromatography. Results: I cylindrica root ethanol extract had a total polyphenol content of 1.109% gallic acid equivalent, total flavonoid content of 0.1% quercetin equivalent, and antioxidant activity IC50 824.30 μg/ml. Sinensetin and Imperatorin were also identified in Fractions 1 to 11 with concentrations of 0.0157 and 0.0178 mg/kg extract, respectively. Conclusion: I. cylindrica root ethanol extract from Sragen had active phytochemical compounds of polyphenols, flavonoids, and antioxidants as well as Sinensetin and Imperatorin.

10aAntioxidant10aFlavonoid10aImperata cylindrica10aPolyphenol1 aIndriyanti, Raden, Anita1 aAriyanto, Eko, Fuji1 aUsman, Hermin, Aminah1 aEffendy, Ristaniah, Rose1 aDhianawaty, Diah uhttps://www.phcogj.com/article/1838