02601nas a2200253 4500008004100000245016300041210006900204260001600273300001200289490000700301520174900308653000902057653001602066653002802082653002202110653001202132653001802144100003102162700002602193700002902219700003502248700002402283856004002307 2022 eng d00aChemical Profiling, Antioxidant and Lipoxygenase Enzyme Inhibition Activities of Wild Edible Truffle (Terfezia boudieri) from Northern Borders of Saudi Arabia0 aChemical Profiling Antioxidant and Lipoxygenase Enzyme Inhibitio cAugust 2022 a319-3260 v143 a

Truffles are natural food product very famous for its health benefits for being significant biosource of essential fatty acids, proteins and other antioxidant and phenolic compounds. The current study was conducted to evaluate the phytochemicals, antioxidant and lipoxygenase inhibition activities of Terfezia boudieri of Saudi origin. Various phytochemicals were screened applying standard procedures. The total methanol extract (TME) of the truffle was subjected to several chromatographic procedures. The antioxidant activity was evaluated by DPPH antioxidant procedure, comparing results with trolox as standard. Results demonstrated that Terfezia boudieri chemically characterized by the availability of various constituents such as flavonoids, steroids, saponins, tannins and carbohydrates at different levels. Phytochemical investigation led to the isolation of β-sitosterol and gallic acid that were identified using 1H, 13C, DEPT, COSY, HMQC and HMBC NMR spectroscopic data. Results demonstrated high antioxidant activity with IC50: 50.4 μg/ml and 31.4 μg/ml for TME and gallic acid, respectively. TME and gallic acid exhibited lipoxygenase inhibitory activity with IC50 values 4.59 and 0.53 μg/ml for TME and gallic acid, respectively. The higher lipoxygenase inhibitory activity was presumably correlated to the high antioxidant activity. In conclusion, current investigation confirms the folklore use of Terfezia boudieri as antinflammatory food. Hence, the studied Terfezia boudieri may have a great potential as antioxidant and antinflammatory functional food and nutraceuticals products for pharmaceutical applications.

10aDPPH10aGallic acid10aLipoxygenase inhibition10aTerfezia boudieri10aTruffle10aβ-sitosterol1 aAlshawush, Marwan, Mohamed1 aBurshed, Hussein, Ali1 aAlasoom, Abdullah, Jalal1 aAltaweel, Abdullah, Abdulhamid1 aKhalil, Hany, Ezzat uhttps://www.phcogj.com/article/1826