02380nas a2200241 4500008004100000245011700041210007000158260001400228300001200242490000700254520167500261653001101936653002701947653001101974653000901985653002501994653003002019100002102049700000502070700001802075700000502093856004002098 2022 eng d00aIn Vivo Studies of Combined Probiotics on IFN-γ, Ig-E and Bronchial Muscular Layer of Rats with Allergic Asthma0 aIn Vivo Studies of Combined Probiotics on IFNγ IgE and Bronchial cJune 2022 a598-6030 v143 a

Background: Leuconostoc mesenteriodes and Lactobacillus brevis are being used as probiotic to improve the immune system. The effect of probiotic containing combination of the 2 bacteria on enhancing innate and adaptive immune responses is not fully understood. Objective: This was conducted to analyse the effect of probiotic containing in single or combination content of L. mesenteriodes (Lm) and L. brevis (Lb) on IFN-γ concentration, Ig-E concentration, and histopathology of bronchial muscular layer of ovalbumininduced allergic asthma. Materials and Methods: A total of 40 male Sprague Dawley rats (6–8 weeks, 200-300 g) were randomly divided into 5 groups: NC (non-induced control group); NgC (OVA-induced control group), Lm (OVA +probiotics containing L. mesenteriodes), Lb (OVA + probiotics containing L. brevis), and Lm + Lb (OVA+probiotics containing combination of L. mesenteriodes and L. brevis). On 64-day, concentration of IFN-γ and Ig-E in serum were measured. Histology of bronchus was performed. Results: IFN-γ concentration, Ig-E The administration of single or combined probiotics increased IFN-γ (p<0.001), yet decreased IgE (p<0.001) of all treatment groups, but only a combination of both probiotics reduced the thickness of the bronchial epithelium. Conclusion: The combination of Lm and Lb single or combined probiotics improve systemic and local anti-inflammation effects and ameliorate airway remodelling in the ovalbumin-induced chronic asthma rat model.

10aAsthma10aBronchus histopatology10aIFN-γ10aIg-E10aLactobacillus brevis10aLeuconostoc mesenteriodes1 aPujiati, Pujiati1 a1 aIndarto, Dono1 a uhttps://www.phcogj.com/article/1807