01828nas a2200229 4500008004100000245018000041210006900221260001400290300001200304490000700316520107900323653001301402653000901415653001701424653002001441653001001461100002401471700002401495700002001519700001901539856004001558 2022 eng d00aEffective Enzyme-Producing Bacteria Isolated from Diversified Thai Rice and Native Thai Bees (Cavity Nesting Honey Bees) and their Potential for Production of Protease Enzymes0 aEffective EnzymeProducing Bacteria Isolated from Diversified Tha cJune 2022 a506-5170 v143 a

Background: Thailand is one of the most bio-diversified countries in Southeast Asia, especially regarding plants, animals and microbes that impact directly on human and animal life. Methods: Of the 201 bacterial isolates that were screened from Thai rice and bees in Ratchaburi and Chiang Mai provinces, Thailand, most were classified in the genus Bacillus. Result: From the morphological test and 16S ribosomal DNA sequence study, three strains, Bacillus flexus KRptI_S2, Microbacterium paraoxydans C13HN2 and Paenibacillus hunanensis KRrb_T2, could produce amylase and protease enzyme and non-hemolytic activity at higher temperature. The highest enzymatic activity of protease was produced by P. hunanensis KRrb_T2 (378.9967±1.335 U.mL-1) at 36 h. Conclusion: In summary, the bacteria from Thai rice and bees could be potential suitable sources of protease production for development by the food and beverage industries globally.

10aBacteria10aBees10aBiodiversity10aProtease Enzyme10aRice.1 aChumphon, Thapakorn1 aPromnuan, Yaowanoot1 aMeelai, Sujinan1 aPromsai, Saran uhttps://www.phcogj.com/article/1796