02368nas a2200217 4500008004100000245020600041210006900247260001400316300001200330490000700342520161500349653002201964653001501986653001002001100002902011700001602040700002802056700000502084700002102089856004002110 2022 eng d00aToll Like Receptor 4 Expression Profile in Mice Infected Mycobacterium Tuberculosis Given with Miana Leaves Extract (Coleus scutellarioides (L.) Benth) (Tuberculosis Preventive and Curative Mechanisms)0 aToll Like Receptor 4 Expression Profile in Mice Infected Mycobac cJune 2022 a497-5050 v143 a

Introduction: Tuberculosis is an infectious disease of the respiratory tract caused by Mycobacterium tuberculosis. During Mycobacterium tuberculosis infection, pathogens modulate TLR-4 receptor flow signaling, suggesting possible involvement of TLR-4 in the regulation of the host immune response. This study aims to determine the effect of miana leaf extract on the expression of toll like receptor 4 (TLR-4) in tuberculosis mice at the preventive and curative stages. Methods: Mice were divided into 3 groups with 7x replication. Providing 14 days of preventive treatment, 14 days of incubation and 14 days of curative treatment. Group 1 and group 3 were given Miana Leaves Extract (EDM) and placebo at all three stages. Group 2 was given EDM at the preventive and incubation stages, then they were given anti-tuberculosis drugs (OAT). Results: The results proved that EDM given as a preventive did not increase the expression of TLR-4 protein in healthy mice; Changes in expression of TLR-4 protein in M.tb-infected mice before and after curative EDM increased by 17%, after administration of placebo increased 97% and decreased 12% after OAT curative administration; Changes in expression of TLR-4 protein in M.tb-infected mice before preventive administration and after EDM curative administration increased by 20%, after administration of placebo increased 102% and decreased by 10% after the curative administration of OAT. Conclusions: EDM has potential as antituberculosis with TLR-4 regulatory mechanism.

10aAntituberculosis.10aMiana Leaf10aTLR-41 aPakadang, Sesilia, Rante1 aRatnah, St.1 aSalasa, Alfrida, Monica1 a1 aHatta, Mochammad uhttps://www.phcogj.com/article/1795