02185nas a2200217 4500008004100000245016400041210006900205260001500274300001200289490000700301520142800308653000901736653001701745653007801762653001001840653001001850100000501860700003301865700002901898856004001927 2022 eng d00aCytotoxic Effects of Kirinyuh Herb (Austroeupatorium inulaefolium (Kunth) R. d. King & H. Robinson) Extracts and Fractions on BSLT, MCF-7 Cells and T-47D Cells0 aCytotoxic Effects of Kirinyuh Herb Austroeupatorium inulaefolium cApril 2022 a374-3780 v143 a

Breast Cancer is the second-highest prevalence of cancer disease in Indonesia, with the number of new cases and deaths continues to increase. Kirinyuh or Austroeupatorium inulaefolium (Kunth) R. D. King & H. Robinson is a shrub plant, which are widely grown in Indonesia. Traditionally, it is used for woundhealing and information about anticancer potential of A. inulaefolium is still very limited. This study aims to assess the potential of cytotoxic activity of A. inulaefolium on MCF-7 and T-47D breast cancer cells. Tests that carried out in this study were phytochemicals screening, Brine Shrimp Lethality Test (BSLT) and Cytotoxicity assay. The phytochemicals screening showed that EA (leaves extracts levels 1-3), EB (leaves extracts levels 4-6) and EC (stalk extracts) contained tannin, steroids/triterpenoids, flavonoids and phenols. The BSLT results showed that the EB has the smallest LC50 value of 12.86 μg/mL. MTT assay results showed the smallest IC50 value of the extract and the fraction on MCF-7 cells were EA 204.96 μg/ mL and FEA 205.43 μg/mL and T-47D cells were EB 217.03 μg/mL and FEA 192.36 μg/mL. The conclusion of toxicity and cytotoxicity of the herb (leaves and stalks) ethanol 96% extracts of A. inulaefolium had a very toxic effect on BSLT however had less potential cytotoxicity on MCF-7 cells and T-47D cells.

10aBSLT10aCytotoxicity10aKirinyuh (Austroeupatorium inulaefolium (Kunth) R. D. King & H. Robinson)10aMCF-710aT-47D1 a1 aSoemardji, Andreanus, Andaja1 aTan, Marselina, Irasonia uhttps://www.phcogj.com/article/1777