02101nas a2200193 4500008004100000245014900041210006900190260001500259300001200274490000700286520143600293653001701729653002301746653002401769100001701793700003301810700002401843856004001867 2022 eng d00aFormulation and Characterization of Meniran (Phyllanthus Niruri Linn) Extract Nanoparticle on Antibacterial Activity Against Salmonella Pullorum0 aFormulation and Characterization of Meniran Phyllanthus Niruri L cApril 2022 a369-3730 v143 a

This study aims to examine the results of meniran extract (Phyllanthus niruri Linn.) on antibacterial activity. Salmonella Pullorum is capable of causing huge economic losses. The misuse of antimicrobials has resulted in the evolution of multidrug-resistant strains. Meniran has potential as an antibacterial because it contains many bioactive components such as alkaloids, flavonoids, tannins and saponins. Nanoparticles help in the bioavailability of plant extracts. The research was conducted by making a meniran extract nanoparticles formulation with ionic glass method using chitosan and TPP sodium with a dose difference of 5%, 10% and 20%. Each dose of meniran nanoparticles then were characterized by PSA, SEM and TEM. The result on PSA showed that size range from 192.67 nm to 385.16 nm and 5% meniran extract nanoparticles have the best homogeneity and stability. EE value showed that the increase in the dose was directly proportional to the increase in the EE value. The result on SEM showed that the overall production of nanoparticle samples, it looks like they are nano-sized. The result on TEM showed small sample morphology with a good distribution. After that, the antibacterial activity test was then carried out using the MIC and MBC tests. The results showed that 5% of meniran extract nanoparticles had the best antibacterial activity against Salmonella Pullorum.

10aNanoparticle10aPhyllanthus niruri10aSalmonella Pullorum1 aHidanah, Sri1 aSabdoningrum, Emy, Koestanti1 aSudjarwo, Sri, Agus uhttps://www.phcogj.com/article/1776