02012nas a2200157 4500008004100000245012100041210006900162260001800231300001000249490000700259520149000266100001801756700002001774700002001794856004001814 2022 eng d00aImproving Enzyme-Assisted Extraction of Brazilin from Sappanwood (Caesalpinia Sappan L.) Extract by Fungal Cellulase0 aImproving EnzymeAssisted Extraction of Brazilin from Sappanwood  cFebruary 2022 a21-280 v143 a

Brazilin was one of phytoconstituent from sappanwood that widely used as textile-colouring agent and found in traditional concoction for therapeutic purposes. Due to obtain its maximum level in sappanwood (Caesalpinia sappan L.) extract, the extraction method of brazilin has been developed. Enzyme-assisted extraction (EAE) is one of green chemistry methods to achieve that outcome. This study aims to enhance brazilin level by optimize the EAE condition with fungi cellulase. The cellulase are produced by monoculture Aspergillus niger UICC371 in carboxymethyl cellulose submerged fermentation’s media. Sappanwood extracted with fungi cellulase through variation conditions: enzyme concentrations (2.0; 4,0; 6.0%); temperature (45, 50, 55℃); and time (1, 2, 3 hrs). The optimization are provided by response surface method-BoxBehnken design and brazilin level was carried out through High Performance Liquid Chromatography (HPLC) with asetonitril : 0,3% acetic acid in water (14.5 : 85.5) as eluents. The study showed that cellulase from monoculture of Aspergillus niger UICC371 showed optimum condition of cellulase-EAE method at 6.0% concentration enzyme at 50℃ for 3 hours extraction time which provide an increase in brazilin level to 5.014% compare to reflux method.

Key Words: Caesalpinia sappan L., Cellulase, Enzyme assisted extraction, Fungi, Response surface method.

1 aSeptiani, Dia1 aSuryadi, Herman1 aMun’im, Abdul uhttps://www.phcogj.com/article/1732