02237nas a2200217 4500008004100000245017400041210006900215260001800284300001400302490000700316520146600323653003701789653002101826653004001847653001101887100001801898700002201916700002301938700001801961856004001979 2021 eng d00aAnalyzing of Urine 3-Hidroxy Propil Mercapturic Acid on Cyclophosphamide Induced Rat to Determine Ameliorating Effect of Leucaena leucocephala (Lam.) de Wit Seed Extract0 aAnalyzing of Urine 3Hidroxy Propil Mercapturic Acid on Cyclophos cDecember 2021 a1549-15560 v133 a

Background: A 70% ethanolic extract of Leucaena leucocephala (Lam.) de Wit seeds contain a certain amount of sulfhydryl active compounds and potential for ameliorated cyclophosphamide side effects. Objective: The aim of this study was to analyze of urine 3-hidroxy propil mercapturic acid (3-HPMA) in a rat model for cyclophosphamide-induced hemorrhagic cystitis to determine the effect of Leucaena leucocephala (Lam.) de Wit seeds extract. Materials and methods: The levels of urine 3-hidroxy propil mercapturic acid was measured using LCMS/MS. Urine preparation was carried out by dilution (1:5) with mobile phase and contained a deuteurated internal standard. The LC separation was performed using a C18 column (1.7 μm; 2.1 mm × 100 mm). The mobile phase consisted of formic acid (0.1%) and formic acid (0.1%) in acetonitrile (90:10 v/v), as a starting gradient at flow rate of 0.2 ml/min with total run 7 min. Results: The lower limit of quantification was 40 ng/ml and the linear extended to 20,000 ng/ml. The method has been successfully to be applied in determined of rat urine 3-HPMA. The results showed that the 70% ethanolic extract of seeds can increased the amount of 3-HPMA in rat urine. Conclusion: The 70% ethanolic extract of seeds displayed a potential ameliorating effect against cyclophosphamide side effects.

10a3-Hidroxypropil mercapturic acid10aCyclophosphamide10aLeucaena leucocephala (Lam.) de Wit10aUrine.1 aWardatun, Sri1 aHarahap, Yahdiana1 aSutandyo, Noorwati1 aMun'im, Abdul uhttps://www.phcogj.com/article/1697