02227nas a2200265   4500008004100000245014700041210006900188260001800257300001400275490000700289520140600296653002101702653001501723653003601738653002101774653001401795653001601809653001501825100000501840700001901845700002201864700001901886700001601905856004001921       2021                        eng d00aImmunostimulant Activity of Pegagan Embun Herbs Extract (Hydrocotyle sibthorpioides Lam.) With Carbon Clearance Method Towards Male White Mice0 aImmunostimulant Activity of Pegagan Embun Herbs Extract Hydrocot  cNovember 2021  a1472-14770 v133 a<p class="rtejustify">Pegagan embun (<em>Hydrocotyle sibthorpioides </em>L.) has been used traditionally to cure various diseases such as anti-inflammatory, neutralizing toxins, dysentery, and others. This study aims to determine the immunostimulant effect of pegagan embun herbs extract using the carbon clearance method, the number, and the percentage of leukocyte cells. In this study, 25 male white mice were used, which were divided into five groups, groups 1, 2, and 3 given pegagan embun herbs extract in a dose of 10 mg/kgbw; 50 mg/ kgbw; 200 mg/kgbw, group 4 was given 0,5% Na CMC, and group 5 were given stimuno 50 mg/kgbw. The preparation is given orally for seven consecutive days. On the eighth day, the phagocytosis index, number, and percentage of leukocyte cells were determined. The results showed that pegagan embun herbs extract has immunostimulant activity. The phagocytosis index of the 10 mg/kgbw dose group is 1.0211, 1.27796 for the 50 mg/kgbw, and 1.5250 for the 200 mg/kgbw. Then, the number of leukocytes of the 10, 500, and 200 mg/kgbw are 8.820/μL, 9.906/μL, and 10.790/μL, respectively. The percentage of lymphocyte cells are 58,5%, 60,6% and 62,8%, and for segment neutrophil cells were 26%, 27,6% and 30% for group 10, 50, and 200 mg/kgbw extract. Based on the results, that the pegagan embun herbs extract has immunostimulant activity against male white mice.</p>
10aCarbon clearance10aEosinophil10aHydrocotyle sibthorpioides Lam.10aImmunostimulants10aLeukocyte10aLymphocytes10aNeutrophil1 a1 aFitri, Afdhila1 aHusni, Elidahanum1 aBadriyya, Elsa1 aAldi, Yufri  uhttps://www.phcogj.com/article/1684