02324nas a2200241 4500008004100000245014100041210006900182260001800251300001400269490000700283520155100290653001701841653001001858653002801868653001401896653001401910100002301924700002201947700002401969700002601993700002302019856004002042 2021 eng d00aThe Effect of Sambiloto and Spirulina Combination on Mucin-1 Protein Expression in Medial Colon of Plasmodium berghei ANKA Infected Mice0 aEffect of Sambiloto and Spirulina Combination on Mucin1 Protein  cNovember 2021 a1359-13650 v133 a

Malaria still be health problem in the world, especially in Eastern Indonesia. Malaria’s inflammation and metabolism defect can cause colonic damage, such as enhancement Muc-1 protein expression and goblet cells hyperplasia. Sambiloto and spirulina combination as antiinflammatory and antioxidative agent can prevent medial colon damage Plasmodium berghei ANKA infected mice. The aim of the study to show the effect of sambiloto and spirulina combination on Muc-1 protein activity in medial colon Plasmodium berghei ANKA infected mice. This study use preserve male Swiss Webser mice colonic tissue which has inoculated by Plasmodium berghei ANKA, whose treatment group include positive control (dehyroartemisin piperaquine), negative control (carboxymethil cellulose), AP (sambiloto), AP+ES (sambiloto+spirulina extract), and AP+PS (sambiloto+spirulina powder) and terminated after 28 days of treatment. Colonic tissue was stained with immunohistochemistry and observed using light microscope (400x) in five different field and was analyzed with ImageJ® sowtware, and statisitcal analysis was done with SPSS 20.0. According to One Way ANOVA and Duncan posthoc test, only AP+PS(120,98 ±3,37), which significantly difference between negative control, AP, and AP+ES group. Meanwhile, between DHP, AP+PS group not significantly differenece. Sambiloto extract and spirulina powder combination can reduce Muc-1 protein expression in medial colon Plasmodium berghei ANKA infected mice.

10aMedial colon10aMuc-110aPlasmodium berghei Anka10aSambiloto10aSpirulina1 aKusmardi, Kusmardi1 aHairi, Baiqi, Nur1 aLubis, Nadar, Sukri1 aLestari, Tri, Wahyuni1 aIntan, Putri, Reno uhttps://www.phcogj.com/article/1670