02625nas a2200217 4500008004100000245017700041210006900218260001800287300001400305490000700319520189400326653002902220653000802249653001702257653001902274100001602293700001802309700001602327700002402343856004002367 2021 eng d00aAnti-Inflammatory Effects of Methanol Extract, Hexane, Ethyl Acetate, and Butanol Fraction of Piper crocatum Ruiz & Pav Leaves on Lipopolysaccharide-induced RAW 264.7 Cells0 aAntiInflammatory Effects of Methanol Extract Hexane Ethyl Acetat cNovember 2021 a1341-13460 v133 a

Piper crocatum Ruiz & Pav is a traditional Indonesian plant that is empirically used to treat various diseases. Several studies have stated that Piper crocatum leaves extract has anti-inflammatory effects. Piper crocatum leaves contain different secondary metabolites alkaloids, flavonoids, tannin-polyphenols, steroids, terpenoids, and saponins. The purpose of this study was to determine the anti-inflammatory effect of Piper crocatum leaves extract and fraction on the production of nitric oxide in lipopolysaccharideinduced RAW 264.7 cells. Anti-inflammatory effect of methanol extract of Piper crocatum leaves (MEPC), n-hexane fraction of Piper crocatum leaves (HFPC), ethyl acetate fraction of Piper crocatum leaves (EAFPC), and n-butanol fraction of Piper crocatum leaves (BFPC) against RAW 264 cell macrophages, 7 Lipopolysaccharide-induced (LPS). Extraction of Piper crocatum leaves was the maceration method. Cell viability was determined by the MTT method in the concentration range of 12.5;25;50;100;200 ug/ mL. The anti-inflammatory effects of MEPC, HFPC, EAFPC and BFPC were tested against nitric oxide (NO) production inhibition in LPS-induced RAW 264.7 macrophage cells. NO levels were determined by the NO Colorimetric Assay Kit as measured by the ELISA plate reader. The viability test of MEPC, HFPC, EAFPC, and BFPC from Piper crocatum leaves showed the concentrations that gave the percentage of viability above 80% were concentrations of 100, 50, and 25 g/mL. MEPC, HFPC, EAFPC, and BFPC at concentrations of 100, 50, 25 μg/mL could significantly inhibit NO production with p<0.05 (p=0.000). The highest NO production inhibition effect was on EAFPC. MEPC, HFPC, EAFPC, and BFPC have antiinflammatory effects, with the highest effect on EAFPC.

10aAnti-inflammatory Effect10aLPS10aNitric oxide10aPiper crocatum1 aLely, Nilda1 aArifin, Helmi1 aAldi, Yufri1 aWahyuni, Fatma, Sri uhttps://www.phcogj.com/article/1667