02955nas a2200265 4500008004100000245012600041210006900167260001900236300001400255490000700269520207500276653001602351653002202367653001502389653002102404653001702425653002102442100003402463700003202497700002902529700003102558700002702589700003302616856004002649 2021 eng d00aAntioxidant Capacity of Chuquiraga Spinosa Less. "Huamanpinta" and Prevention of Carrageenan-Induced Inflammation in Mice0 aAntioxidant Capacity of Chuquiraga Spinosa Less Huamanpinta and  cSeptember 2021 a1287-12960 v133 a

Objective: To evaluate the antioxidant capacity of Chuquiraga spinosa extracts and prevention of carrageenan-induced inflammation in mice. Methodology: Experimental design: plant species, erythrocytes and male BALB C53 mice, were considered as biological material. Antioxidant capacity was evaluated in 50%, 70%, 96% and aqueous ethanolic extracts by 2,2-Diphenyl-1-Picrylhydrazyl reduction, malondialdehyde inhibition in oxidized erythrocytes with H2O2 and correlating with polyphenol content equivalent to gallic acid/gram dry extract. Inflammation was evaluated by inoculating carrageenan 2% in "subcutaneous air bag" of mice: 1) White, 2) carrageenan, 3) dexamethasone 2 mg/kg, 4-6) ethanolic extract 70% doses 100, 250 and 500 mg/kg respectively; determining nitric oxide, malondialdehyde, total proteins, albumin, leukocytes in exudate and histological changes. Results: Alkaloids, flavonoids, terpenes, phenolic compounds, tannins, carbohydrates, triterpenes, steroids and sesquiterpene lactones were identified; aqueous extract presented greater reduction of 2,2-Diphenyl-1-Picrylhydrazyl (CI50 = 58.99 μg/mL), ethanolic extract 70% presented greater inhibition of malondialdehyde in erythrocytes (CI50 = 16.44 nm/mL); It was observed that the higher the amount of polyphenols, the greater the reduction of 2,2-Diphenyl-1-Picrylhydrazyl (r=-0.909) and the greater the inhibition of malondialdehyde (r=-0.781). With 500 mg/kg of 70% ethanolic extract there was greater anti-inflammatory effect inhibiting malondialdehyde, nitric oxide, albumin, total proteins and leukocytes in 55.55%, 81.92%, 41.20%, 31.51% and 32.45% (p<0.01) respectively and less infiltration of leukocytes and lymphocytes in air sac membrane. Conclusion: The extracts of aerial parts of Chuquiraga spinosa showed antioxidant capacity correlated to polyphenol content. The 70% ethanolic extract prevented inflammation in mice in a dosedependent manner.

10aAntioxidant10aEthanolic extract10aLeukocytes10aLipoperoxidation10aNitric oxide10aOxidative stress1 aJustil-Guerrero, Hugo, Jesús1 aArroyo-Acevedo, Jorge, Luis1 aRojas-Armas, Juan, Pedro1 aPacheco, Miriam, Palomino-1 aVillena-Tejada, Magaly1 aVílchez, Wilmer, Atilio Seg uhttps://www.phcogj.com/article/1660