02745nas a2200313 4500008004100000245012400041210006900165260001900234300001400253490000700267520167800274653002901952653003101981653002502012653002402037653000902061653002802070653002002098100004102118700002602159700002502185700003202210700002602242700002602268700002502294700003202319700004002351856004002391 2021 eng d00aPhytochemical Screening and Anti-Inflammatory Potential of the Organic Extracts from Cleoserrata serrata (Jacq.) Iltis.0 aPhytochemical Screening and AntiInflammatory Potential of the Or cSeptember 2021 a1225-12410 v133 a

Cleoserrata serrata is used in Mexican-south traditional medicine to treat chicleros ulcer. The phytochemical profile and the anti-inflammatory effect from four extracts obtained by maceration proccess and of the primary fractions from two extracts are described. In addition, the antioxidant, leishmanicidal and antimycobaterial activities and LD50 from active extracts are reported. Anti-inflammatory activity was evaluated in TPA and carrageenan assays. Ethyl palmitate and γ-sitosterol were detected in Hexanic and CH2Cl2 extracts. Same compounds and stigmasta-3,5-dien-7-one, palmitic acid, phytol acetate and phytol were detected in primary fractions from CH2Cl2:EtOH extract. In this and in MeOH extracts a polyphenolmixture was obtained. The MeOH extract was subjected to acid hydrolysis, and kaempferol, quercetin and scopoletin were detected in organic-phase. Polyphenol-mixture and organic-phase (IC50=3730 and 2338 μg/mL) showed moderate antioxidant activity; meanwhile MeOH extract exhibited scarce activity. In carrageenan model, Hexanic extract and polyphenol-mixture showed ED50=131.46 and 64.89 mg/ kg, respectively. Three extracts were active but not-dose-dependent. In TPA-model, CH2Cl2 extract and polyphenol-mixture showed ED50 <0.79 mg/ear, and three extracts were active, however the effect was not-dose-dependent. CH2Cl2:EtOH showed antimycobacterial and leishmanicidal activities. The LD50 was >2 g/kg for all extracts.

10aAnti-inflammatory Effect10aAntimycobacterial activity10aantioxidant activity10aCleoserrata serrata10aDL5010aLeishmanicidal activity10aOrganic extract1 aJuárez-Vázquez, María, del Carmen1 aA, Alejandro, Zamilpa1 aLeón-Díaz, Rosalba1 aMartínez-Vázquez, Mariano1 aLópez-Torres, Adolfo1 aLuna-Herrera, Julieta1 aYépez-Mulia, Lilian1 aAlarcón-Aguilar, Francisco1 aJiménez-Arellanes, María, Adelina uhttps://www.phcogj.com/article/1653