02416nas a2200229 4500008004100000245017500041210006900216260001900285300001400304490000700318520164000325653002401965653001601989653001502005653001102020100002102031700003302052700002702085700002902112700000502141856004002146 2021 eng d00aSeparation and Quantification of Sinensetin, Imperatorin and Total Tannin Content as Active Phytoconstituents of Methanol Extract of Imperata cylindrica Root from Kendari0 aSeparation and Quantification of Sinensetin Imperatorin and Tota cSeptember 2021 a1216-12240 v133 a

Introduction: Imperata cylindrica root methanol extract harvested from Kendari had anti-hypertensive activity stated in a study on hypertension rat models at the doses 60 and 90 mg/kg-bw. The extract also contained phenolic compound 1.53% (GAE) and flavonoid 1.17% (QE) and had antioxidant activity IC50 0.32 mg/mL. Aim of the study was to determine the active secondary metabolites in the extract. Materials and Method: Sinensetin and imperatorin in the extract were separated on silica gel 60 column, eluted with a gradient eluent system as follows (1) n-hexane- ethyl acetate (3:7); (2) n-hexane – ethyl acetate (2:8); (3) ethyl acetate -MeOH (8:2), and (4) methanol. Imperatorin and sinensetin in fractions were identified and quantified by HPLC method with column LiChroCART 250-4,6 RP 18E, isocratic mobile phases with the composition of mixture as follows, 0.1% formic acid, acetonitrile and methanol (40:50:10) respectively, and at temperature 400 C. The total tannin was determined by spectrophotometry method with Fe(III) chloride and 1.10-Phenanthroline at wave length 510 nm. Results: Imperatorin and sinensetin were identified in fractions 5 to 9. The concentrations of sinensetin and imperatorin in Imperata cylindrica root methanol extract were of 32.348 and 37.014 mg/kg extract, respectively. The total tannin content was 1.06 % (w/w) (TAE). Conclusion: Imperata cylindrica root methanol extract from Kendari had active phytoconstituents, namely sinensetin, imperatorin, and tannin.

10aImperata cylindrica10aImperatorin10aSinensetin10aTannin1 aDhianawaty, Diah1 aSyamsunarno, Mas, Rizky Angg1 aDwiwina, Resti, Gradia1 aIndriyanti, Raden, Anita1 a uhttps://www.phcogj.com/article/1652